To investigate the role of the cyclic adenosine monophosphate-dependent transcription factor gene Cdtf1 in the pathogenesis of the peach brown rot fungus Monilinia fructicola,the wild-type strain Bmpc7 was used to predict the structure and function of MfCdtf1 gene based on genomic and early tran-scriptomic analyses. The target gene was knocked out and subsequently complemented,and phenotype and stress resistance of transformants were measured. The results showed that MfCdtf1 expression var-ied significantly during the early infection stage of M. fructicola. MfCdtf1 expression increased signifi-cantly at 1 h and 24 h after inoculation,suggesting that it was involved in regulation of M. fructicola in-fection on peach fruit. Phenotypic observation of three homozygous MfCdtf1 knockout transformants and three heterozygous complemented transformants showed that the knockout of MfCdtf1 gene re-sulted in reduced mycelial growth rate and virulence of M. fructicola,lost sporulation and pigment syn-thesis abilities. The test of stresses such as glycerol,H2O2,hexadecyl trimethyl ammonium bromide,so-dium dodecyl sulfate,CaCl2,KCl and NaCl showed that the growth rate of ΔMfCdtf1 strains was lower than that of wild-type strain Bmpc7,indicating that the absence of MfCdtf1 reduced the sensitivity to ex-ogenous stresses.
关键词
桃褐腐病/实生链核盘菌/MfCdtf1基因/致病机制/外源胁迫/抗逆性
Key words
peach brown rot/Monilinia fructicola/MfCdtf1 gene/mechanisms of pathogenicity/exogenous stress/stress resistance
维普
万方数据