In order to explore the feasibility of chemical fungicides combined with Bacillus amylolique-faciens X-119 strain to control grape gray mold,the chemical fungicides with good antibacterial effect were screened by plate test and combined with Ba. amyloliquefaciens X-119 strain to determine the con-trol effect of different volume ratios on grape gray mold. The optimal combination was obtained by ob-serving and screening the mycelial morphology,sporulation,nuclear production ability and the expres-sion level of major facilitator superfamily (MFS) related regulatory genes. The results showed that the mixture of fludioxonil and Ba. amyloliquefaciens X-119 at a volume ratio of 8:2 had the best antibacte-rial effect on Botrytis cinerea H259 and H261 strains. The toxicity ratios were 1.77 and 1.73,respective-ly,and the control effects were 90.91％ and 72.33％,respectively. The control effects on detached fruit were 83.06％ and 65.48％,and the control effect in greenhouse was 76.72％ and 76.24％,and the control efficacy of compound agent was higher than that of single agent. The mixture of fludioxonil and Ba. am-yloliquefaciens X-119 strain hindered the normal growth of mycelia and significantly reduced the sporu-lation ability and nuclear production ability of Bo.cinerea. The results of real-time fluorescence quantita-tive PCR showed that the expression levels of cell membrane transporter-related regulatory genes BCIN_09g00960,BCIN_03g02340 and BCIN_09g00210 were significantly down-regulated after the treatment with Bo. cinerea. The results showed that the combination of fludioxonil and Ba. amylolique-faciens X-119 strain showed promising prevention and control efficacy. In production,the mixture of fludioxonil and Ba. amyloliquefaciens X-119 strain at a volume ratio of 8:2 could be used for the pre-vention and control of grape gray mold.
关键词
葡萄灰霉病/解淀粉芽胞杆菌/咯菌腈/复配/毒力测定/作用机理
Key words
grape gray mold/Bacillus amyloliquefaciens/fludioxonil/compound/virulence determination/mechanism of action
维普
万方数据