Genome-wide prediction and functional identification of pathogenic effectors of fungus Streptobotrys caulophylli
In order to explore the pathogenic effectors of fungus Streptobotrys caulophylli,secreted pro-teins predicted based on the amino acid sequences obtained from the strain StreptoBC-1 genomes were analyzed using bioinformatics method.Types of carbohydrate enzymes,virulence related proteins and pathogenic effectors of secreted proteins were also predicted.Ten candidate pathogenicity effector genes of the strain StreptoBC-1 were verified by real-time fluorescence quantitative PCR.The results showed that a total of 9 833 amino acid sequences were obtained,and a total of 483 classical secreted proteins were detected(accounted for 4.9％)including 169 carbohydrate active enzymes(CAZymes),274 annotated virulence-related proteins of Botrytis cinerea,and 26 candidate pathogenicity effectors.It was found that the relative expression levels of nine out of ten randomly selected pathogenicity effec-tors were significantly up-regulated and one down-regulated at 24 h post-inoculation of cucumber leaves;the gene expression ratios were 1.9 to 3 196.8-fold change,and only one was significantly down-regulated compared with the control group.Our results revealed that effectors screened were closely re-lated to the infection process of S.caulophylli,and most of the verified candidate pathogenicity effec-tors that could positively regulate the S.caulophylli infection.
万方数据
维普
