Isolation and whole genome analysis of a pathogenic bacterial strain causing potato blackleg in Wuchuan County,Inner Mongolia Autonomous Region
In order to clarify the pathogenic species responsible for blackleg disease in Wuchuan Coun-ty,Inner Mongolia Autonomous Region,and uncover the pathogenic mechanism,potato plants showing typical blackleg symptoms were collected from this region.Bacteria were isolated from these symptom-atic samples,and their pathogenicity was assessed using tuber and plant inoculation methods.The patho-genic bacterial isolate was identified with morphological characters,16S rRNA sequence analysis,and comparative complete genome sequence analysis.The complete genome sequence of pathogenic strain was determined by the Pacbio Sequel Ⅱ,and then the potential pathogenic mechanisms was predicted.Results showed that one pathogenic isolate,named TDHJ7,was isolated from symptomatic plants,and it was identified as Pectobacterium atrosepticum based on morphological characteristics,16S rRNA anal-ysis,and comparative complete genome sequencing.The whole genomic sequence of P.atrosepticum TDHJ7 was 4 997 951 bp,containing 4 623 coding sequences.Pathogenic annotation of strain TDHJ7 showed that 245 genes were involved in the pathogenic process,among them,22,19,and 12 genes were involved in the synthesis of pectinase,cellulase,and protease,respectively 171 genes were involved in type Ⅰ,Ⅱ,Ⅲ,Ⅳ,and Ⅵ secretion systems,and 21 genes were involved in annotating toxin.The results suggest that the potential pathogenicity mechanisms of the strain TDHJ7 included cell wall-degrading enzymes(such as pectinase,cellulase and protease),secretion systems(including types Ⅰ,Ⅱ,Ⅲ,Ⅳ andⅥ)and toxic compounds.
Inner Mongolia Autonomous Regionpotato backlegisolation and identificationwhole genomePectobacterium atrosepticumpathogenic mechanism
万方数据
维普
