首页|酸模根际解淀粉芽胞杆菌SH-53发酵条件优化及其生防作用

酸模根际解淀粉芽胞杆菌SH-53发酵条件优化及其生防作用

Optimization of fermentation conditions and biocontrol effect of Bacillus amyloliquefaciens SH-53 from rhizosphere of rumex

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为探索解淀粉芽胞杆菌Bacillus amyloliquefaciens SH-53的优化发酵条件及其生防作用,以发酵液OD600nm值和抑菌率为指标,设计单因素试验和响应面试验优化发酵条件,以室内盆栽试验检测优化发酵液对番茄枯萎病防效、番茄苗促生效果及根系防御酶活的影响,并测定菌株SH-53在番茄根系的定殖能力.结果显示:菌株SH-53优化后的发酵条件为蔗糖15.00 g/L、硝酸钠15.00 g/L、磷酸氢二钠5.25 g/L、酵母浸粉5.00 g/L、pH 7.5、温度30℃、转速200 r/min,装液量200 mL培养24h;优化后SH-53发酵液OD600nm值及抑菌率分别较优化前显著提升183.01％和14.06％;在保护试验和治疗试验中防效分别为84.44％和64.86％,能显著提高番茄苗株高、茎粗和根长等农艺性状,可增强番茄根系过氧化物酶、过氧化氢酶、多酚氧化酶和超氧化物歧化酶活性.菌株SH-53在番茄苗根系表面定殖量为1.25×108 CFU/mm.表明解淀粉芽胞杆菌SH-53优化发酵后对番茄枯萎病防治效果良好,可在番茄植株根系定殖,促进植株生长.
To explore the optimal fermentation conditions and biocontrol efficacy of Bacillus amyloliq-uefaciens SH-53,single-factor and response surface tests were designed and performed to optimize the fermentation condition with the OD600nm and the inhibition rate as the indexes.The control efficacy of the optimized fermentation broth on tomato Fusarium wilt,the growth-promoting effect of tomato seed-lings and effect on the activity of root defense enzymes were tested using pot experiments in the labora-tory.The colonization ability of the strain SH-53 on tomato roots was also detected.The results showed that the optimal fermentation conditions of strain SH-53 were as follows:sucrose 15.00 g/L,Na-NO3 15.00 g/L,Na2HPC4 5.25 g/L,yeast extract 5.00 g/L,pH 7.5,30 ℃,200 r/min,cultured in 200 mL liquid for 24 h.After optimization,the OD600nm value and inhibition rate of the fermented broth of strain SH-53 were increased by 183.01％and 14.06％,respectively.The control efficacy of the optimal fermentation broth in the protection and treatment tests was 84.44％and 64.86％,respectively.The opti-mal fermentation broth could significantly improve the plant height,stem diameter,root length and oth-er agronomic traits.In addition,the activities of peroxidase,catalase,polyphenol oxidase and superox-ide dismutase also increased in tomato roots.The colonization amount of strain SH-53 on the root sur-face of tomato seedling was 1.25x108 CFU/mm.The results indicated that Bacillus amyloliquefaciens SH-53 had good control efficacy on tomato Fusarium wilt after optimized fermentation,and it could colonize tomato roots to promote plant growth.

Bacillus amyloliquefaciensoptimization of fermentation conditiontomato Fusarium wiltroot colonizationroot defense enzyme

王楠、廖永琴、申云鑫、赵江源、陈齐斌、杨佩文

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云南农业大学植物保护学院,昆明 650201

云南省农业科学院农业环境资源研究所,昆明 650200

云南省微生物研究所,昆明 650091

解淀粉芽胞杆菌 发酵条件优化 番茄枯萎病 根系定殖 根系防御酶

云南省科技计划项目云南省科技计划项目云南省科技计划项目云南省专家基层科研工作站-杨佩文专家工作站

202301BD070001-131202202AE090010202202AE090015云人社通[2022]17号

2024

10.13802/j.cnki.zwbhxb.2024.2024005

植物保护学报
中国植物保护学会 中国农业大学

植物保护学报

CSTPCD北大核心
影响因子：1.207
ISSN：0577-7518
年,卷(期)：2024.51(4)