Chemical identification of tobacco root exudates and their chemotaxis effects on Ralstonia solanacearum
To investigate the components of tobacco root exudates and their chemotaxis effects on the plant pathogen Ralstonia solanacearum,the components of root exudates of Nicotiana tabacum cultivar Yunyan 87 were identified by non-targeted metabolomics based on liquid chromatography-mass spec-trometry(LC-MS).The chemotaxis of R.solanacearum to tobacco root exudates was determined by capillary-like stucture test,and soil-degrading bacteria were screened by using the attractant of R.sola-nacearum as the sole carbon source.The results showed that 655 compounds were detected by LC-MS,including lipids and lipid-like molecules,nucleosides,nucleotides and analogues,benzenoids,phenyl-propanoids and polyketides,organic oxygen compounds,alkaloids and derivatives,etc.R.solanacearum had chemotaxis to gallic acid,L-homoserine,stachyose,5-aminosalicylic acid,monolaurin,2-hydroxy-hexanoic acid and 3,3-dimethylglutaric acid.Soil-degrading bacteria Achromobacter marplatensis HS001,Arthrobacter enclensis HS002,Microbacterium azadirachtae HS003,Arthrobacter bambusae DS001 and Pseudomonas nitritereducens DS002 were isolated and could be used to degrade stachyose.
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维普
