摘要
为探究烟草根系分泌物的成分及其对青枯雷尔氏菌Ralstonia solanacearum的趋化性响应,采用基于液相色谱-质谱联用(liquid chromatography-mass spectrometry,LC-MS)技术的非靶向代谢组学分析法对烟草Nicotianatabacum云烟87根系分泌物的组成成分进行鉴定,通过类毛细管试验检测青枯雷尔氏菌对烟草根系分泌物的趋化性,并以青枯雷尔氏菌的引诱剂为唯一碳源进行土壤降解细菌的筛选.结果表明:LC-MS共检测到655种化合物,包括脂类和类脂分子、核苷,核苷酸及类似物、苯类化合物、苯丙烷类和聚酮、有机氧化物、生物碱及其衍生物等.青枯雷尔氏菌对没食子酸、L-高丝氨酸、水苏糖、5-氨基水杨酸、月桂酸甘油酯、2-羟基己酸和3,3-二甲基戊二酸具有趋化性.分离筛选到5株细菌能够降解水苏糖,分别是马德普拉塔无色杆菌Achromobacter marpla-tensis HS001、印度国化室不动杆菌 Arthrobacter enclensis HS002、苦楝树微杆菌 Microbacterium azadirachtae HS003、竹节杆菌 Arthrobacter bambusae DS001 和硝化假单胞菌Pseudomonas nitriter-educens DS002.
Abstract
To investigate the components of tobacco root exudates and their chemotaxis effects on the plant pathogen Ralstonia solanacearum,the components of root exudates of Nicotiana tabacum cultivar Yunyan 87 were identified by non-targeted metabolomics based on liquid chromatography-mass spec-trometry(LC-MS).The chemotaxis of R.solanacearum to tobacco root exudates was determined by capillary-like stucture test,and soil-degrading bacteria were screened by using the attractant of R.sola-nacearum as the sole carbon source.The results showed that 655 compounds were detected by LC-MS,including lipids and lipid-like molecules,nucleosides,nucleotides and analogues,benzenoids,phenyl-propanoids and polyketides,organic oxygen compounds,alkaloids and derivatives,etc.R.solanacearum had chemotaxis to gallic acid,L-homoserine,stachyose,5-aminosalicylic acid,monolaurin,2-hydroxy-hexanoic acid and 3,3-dimethylglutaric acid.Soil-degrading bacteria Achromobacter marplatensis HS001,Arthrobacter enclensis HS002,Microbacterium azadirachtae HS003,Arthrobacter bambusae DS001 and Pseudomonas nitritereducens DS002 were isolated and could be used to degrade stachyose.
基金项目
湖北省烟草公司重点项目(027Y2022-002)
湖北省烟草公司重点项目(027Y2021-017)
维普
万方数据