Control efficacy and action mechanism of ethylicin on apple Valsa canker
To clarify the control efficacy and underlying the action mechanism of ethylicin against apple Valsa canker,the Petri dish-based inhibition of ethylicin on the mycelial growth of Valsa mali was as-sessed using the mycelial growth rate method.The protective and curative effects of ethylicin against Valsa canker were determined through detached branch experiments.Furthermore,the mechanism of ethylicin's influence on conidial germination and mycelial growth was elucidated via microscopic ob-servation and transcriptome analysis.The results indicated that the EC50 of 80％ethylicin emulsifiable concentrate(EC)against the mycelial growth of V mali,the causal agent of apple Valsa canker,was 21.93 μg/mL.The protective and curative effects of 80％ethylicin EC on detached apple branches were 82.20％and 74.73％,respectively,both exceeding those of the two bio-pesticides used as controls.Spe-cifically,the protective and curative effects of the control fungicide,1％phenazine-1-carboxylic acid suspension concentrate,were 48.73％and 63.51％,respectively,while those of 8％ningnanmycin aque-ous solution were 23.29％and 55.00％,respectively.These findings suggest that 80％ethylicin EC ex-hibits excellent control efficacy against apple Valsa canker.Additionally,the incidence of new diseased plants in the two tested apple orchards in Qian and Luochuan counties significantly decreased after the application of 80％ethylicin EC,and control effect of 69.17％and 62.64％against apple Valsa canker,respectively.Further observations revealed that ethylicin markedly inhibited the germination of patho-gen conidia while inducing mycelial malformations and protoplasmic leakage.Moreover,ethylicin sup-pressed the expression of genes related to nutrient metabolism and cell membrane synthesis in the patho-gen,thereby disrupting normal cellular growth.This indicates that ethylicin might exert its antibacterial effect by damaging the cell wall structure and membrane integrity of V mali,inhibiting various enzy-matic activities and metabolic pathways,and ultimately hindering the intracellular energy supply.
control effectapple Valsa cankerValsa malifunction mechanismethylicintranscriptome
万方数据
维普
