Analysis of adult antennal transcriptome and identification of olfaction-related genes in oriental fruit moth Grapholita molesta with different reproductive status
Analysis of adult antennal transcriptome and identification of olfaction-related genes in oriental fruit moth Grapholita molesta with different reproductive status
To determine the effects of different reproductive states on the olfactory genes in the anten-nae of adult oriental fruit moth Grapholita molesta,differentially expressed genes(DEGs)in unmated,gravid,and ovipositional females,as well as unmated,once-mated,and twice-mated males,were ana-lyzed using high-throughput RNA-seq technology.The DEGs were verified by quantitative real-time PCR(qRT-PCR).A total of 19 581 unigenes were obtained,with 16 946 sequences functionally annotat-ed in at least one database,from which 161 olfactory-related genes were identified.Odorant binding proteins(OBPs)and chemosensory proteins(CSPs)showed differential expressions among the three male reproductive states.Pheromone binding protein(PBP)expression was higher in twice-mated males compared to unmated males.In females,compared to unmated and gravid states,odorant receptor(OR)and OBP expressions were upregulated inovipositional females.CSP expression was higher in unmated females than in gravid or ovipositional females.Additionally,PBP and ionotropic receptor(IR)expression levels were higher in ovipositional females compared to unmated females.The qRT-PCR results for differentially expressed OR,OBP,and PBP genes were consistent with the transcriptome sequencing results,indicating the reliability of the transcriptome data in this study.
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