Analysis of spotted alfalfa aphid Therioaphis trifolii salivary gland transcriptome differences based on feeding on susceptible and resistant varieties of Medicago sativa
To clarify the reasons for the differences in fitness of the spotted alfalfa aphid,Therioaphis trifolii,on different varieties of alfalfa,Medicago sativa,salivary glands were collected from two strains of alfalfa spotted aphids:one resistant and one susceptible.These strains had been domesticated from aphid-resistant and susceptible varieties of alfalfa.Transcriptome sequencing was performed using the Illumina second-generation sequencing platform.The sequences were annotated and analyzed to screen differentially expressed genes.Subsequently,eight randomly selected down-regulated differential genes were validated using real-time quantitative PCR(RT-qPCR).The results identified a total of 2 292 dif-ferentially expressed genes in the salivary gland samples of the alfalfa spotted aphid,with 1 191 genes up-regulated and 1 101 genes down-regulated in the salivary glands of the susceptible strain.These genes mainly included those related to nutrient metabolism,signaling pathway transduction,and the synthesis of enzymes related to glucose metabolism.Notably,genes for the degradation of glycopro-teins,glycolipids,oligosaccharides,fucosidase synthesis,and odor recognition were down-regulated.This suggests that these genes might be involved in the counter-defense processes of the alfalfa aphid in response to host plant resistance.The RT-qPCR assay results for the eight differential genes demonstrat-ed a consistent pattern with the transcriptome analysis results,indicating the reliability of the data.It can be hypothesized that the active genes in the salivary glands of the alfalfa spotted aphid,which show greater adaptability to insect-resistant alfalfa,may be key factors involved in the interaction between plants and insects.
万方数据
维普
