Stability analysis and virulence of crude toxin from the mutagenic strain YB4 of Penicillium raistrickii against psyllid Bactericera gobica nymphs
To develop natural microbial-derived insecticides for the control of psyllid Bactericera gobica,the indoor virulence,greenhouse control efficiency,and metabolite composition of crude toxin from Penicillium raistrickii strain F-l and its mutagenic strain YB4 were determined using leaf leaching and leaf spraying methods.The crude toxin with superior virulence was selected,and its stability under different pH,temperature,and UV conditions were measured.The results showed that the indoor toxic-ity and greenhouse control efficiency of the crude toxin from the mutagenic strain YB4 against B.gobica nymphs were higher than those of strain F-l at the same concentration.After 72 h of treatment,the indoor toxicity of the 120 mg/L strain YB4 crude toxin against B.gobica nymphs reached 85.46％.After 14 d of treatment,the greenhouse control effect of the 120 mg/L strain YB4 crude toxin against B.gobica nymphs reached 78.61％.The crude toxins were mainly classified into phenolic acids,alkaloids,flavonoids,terpenoids,quinones,lignans,and coumarins.The optimum pH for the insecticidal action of the YB4 crude toxin is between six and eight,the optimum temperature range is from 25 ℃ to 40 ℃,and the UV irradiation time should not exceed 30 min.These results indicate that the crude toxin from the mutagenic strain YB4 of P.raistrickii had the potential to be developed as a natural microbial insec-ticide.
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维普
