In order to clarify the effects of genetic diversity of plateau zokor Eospalax baileyi popula-tion on grassland ecosystems in the Qinghai-Tibet Plateau,the genetic diversity of plateau zokor popula-tions in Maixi and Hongxing townships in Ruo'ergai County,and Anqu Township in Hongyuan County,Sichuan Province was analyzed with the microsatellite marker technique. The results showed that among the 100 samples analyzed across ten selected microsatellite loci,loci Ectl10 and Ectl22 exhibit-ed the highest allele counts (ranging from 19 to 20),whereas locus Ectl48 had the lowest,with only two alleles. The average number of effective alleles was 4.20,indicating a low genetic diversity. Hardy-Weinberg equilibrium test results indicated a significant imbalance in allele frequencies within the popu-lation,suggesting prevalent inbreeding and restricted gene flow between populations,with limited oppor-tunities for free genetic exchange. Cluster analysis results indicated that genetic exchange between the plateau zokor populations in Ruo'ergai County and Hongyuan County was impeded,with a distant genet-ic relationship facilitated by geographic differences,leading to geographic isolation. Overall,the plateau zokor exhibits low genetic diversity across similar habitats in different geographic locations,with evident genetic differentiation and barriers to gene flow.