Interference with the VmCarEs-6 gene affects the sensitivity of spotted alfalfa aphid Therioaphis trifolii to lambda-cyhalothrin
To elucidate the role of VmCarEs-6 gene in the resistance of the spotted alfalfa aphid The-rioaphis trifolii to high-efficiency cypermethrin,the toxicity of high-efficiency cypermethrin to alfalfa aphids and the relative expression of VmCarEs-6 gene in alfalfa aphids under different lethal concentra-tions of high-efficiency cypermethrin stress were measured indoors. Star polycation (SPc) nanomateri-als were used as carriers to introduce dsVmCarEs-6 into spotted alfalfa aphids,and the interference effi-ciency and survival rate of alfalfa spotted aphids were determined. The indoor toxicity of lambda-cyhalothrin to spotted alfalfa aphids after interference with VmCarEs-6 gene was also measured. The re-sults showed that as the concentration and treatment time of lambda-cyhalothrin increased,its lethal ef-fect on spotted alfalfa aphids became more significant. The lethal concentrations (LC10,LC50,and LC70) of lambda-cyhalothrin against alfalfa aphids were 2.550,5.617,and 7.761 mg/L,respectively. After 48 h of treatment with lethal concentrations of LC10,LC50,and LC70 of lambda-cyhalothrin,the relative ex-pression levels of VmCarEs-6 gene in spotted alfalfa aphids were significantly higher than those in the control group. As the processing time increased,the interference effect of dsVmCarEs-6 became more significant. After 48 h of treatment with dsVmCarEs-6,the relative expression level of the VmCarEs-6 gene in spotted alfalfa aphids was significantly reduced compared to the control group,with an interfer-ence efficiency of 73.30％;After treatment with dsVmCarEs-6 at 6,12,24,36,and 48,the survival rates of spotted alfalfa aphids were 92.22％,88.89％,86.67％,83.33％,and 78.89％,respectively,with no sig-nificant difference compared to the control. However,After 48 hours of treatment with dsVmCarEs-6,the mortality rate in different concentrations of lambda-cyhalothrin treatment groups significantly in-creased compared with the control group (CK). These results indicate that the VmCarEs-6 gene affects the sensitivity of alfalfa aphids to lambda-cyhalothrin.
spotted alfalfa aphidlambda-cyhalothrinRNAitoxicity testingnanometer material
万方数据
维普
