右美托咪定通过调控miR-1307表达对肺癌A549细胞生物学行为的影响
Effect of dexmedetomidine on biological behavior of A549 cells through miR-1307 expession
谢晓梅 1张静 2田行瀚 3于翠翠2
作者信息
- 1. 滨州医学院第二临床医学院,山东烟台 264003
- 2. 烟台毓璜顶医院麻醉科,山东烟台 264003
- 3. 烟台毓璜顶医院重症医学科,山东烟台 264003
- 折叠
摘要
目的 通过检测miR-1307 表达水平,分析右美托咪定(dexmedetomidine,Dex)对肺癌A549 细胞生物学行为影响的可能机制.方法 人肺癌A549 细胞接种后培养 24h,采用数字表法随机分为 4 组:肺癌A549 细胞组、Dex 20μg/ml组、Dex 40μg/ml组及Dex 80μg/ml组;每组每孔设 6 个平行样,各组细胞培养结束后,通过CCK-8 法测定细胞增殖能力,应用Annexin V-FITC/PI双染法观察细胞凋亡,Matrigel 膜及Transwell法测定细胞侵袭力及迁移水平,采用实时荧光定量PCR检测各组细胞中miR-1307 的表达水平.结果 Dex能抑制肺癌A549 细胞活力,且作用呈浓度依赖性及时间依赖性;Dex可诱导肺癌A549 细胞凋亡,当Dex浓度达到 80μg/ml,其凋亡率可升至 22.23%;Dex可抑制肺癌A549细胞的迁移与侵袭能力,且呈浓度依赖性.此外,与对照组比较,Dex处理后A549 细胞中miR-1307 的相对表达量明显下降,且随着Dex浓度的增加下降更为明显.结论 Dex能有效抑制肺癌A549 细胞的增殖、侵袭、迁移,促进其凋亡,并呈剂量依赖性,其作用可能与其调控miR-1307 的表达有关.
Abstract
Objective To analyze the effect of dexmedetomidine(Dex)on biological behavior of A549 cells through expression of miR-1307.Methods Human lung cancer A549 cells were randomly divided into four groups after being cultured for 24 hours:Lung cancer A549 cell group,Dex 20μg/ml group,Dex 40μg/ml group and Dex 80μg/ml group;Each group has 6 parallel samples per hole.After each group of cell culture,we detected the cell proliferation by CCK-8 method,cell apoptosis by flow cytometry,mir-1307 expression by qRT-PCR,cell invasion and cell migration(Transwell)respectively Results Dex inhibits the viability of lung cancer A549 cells in a concentration-and time-dependent manner.Dex can promote the apoptosis of lung cancer A549 cells,and the apoptosis rate can be increased to 22.23%when the concentration of Dex reaches 80μg/ml,the apoptosis rate can rise to 22.23%.Dex inhibits the migration and invasion of lung cancer A549 cells in a concentration-dependent manner.In addition,the relative expression of miR-1307 in A549 cells after Dex treatment decreased significantly comparing to the control group,and the decline was more noteworthy with the increase of Dex concentration.Conclusion Dex can effectively inhibit the proliferation,invasion,metastasis,and apoptosis of humen A549 cells in a dose-dependent manner,and its efficacy may be related to its regulation of miR-1307 expression.
关键词
右美托咪定/miR-1307/肺癌A549细胞/生物学行为Key words
Dexmedetomidine/miR-1307/A549 cells/Biological behavior引用本文复制引用
基金项目
山东省自然科学基金(ZR2020MH197)
出版年
2024
国家科技期刊平台
NSTL
万方数据