Pterostilbene against H2O2-induced hypertrophy of H9C2 cardiomyocytes
Objective To investigate effects of pterostilbene(PTE)on H2O2-induced hypertrophy of H9C2 cardiomyocytes.Methods H9C2 cardiomyocytes were cultured in DMEM,cardiomyocyte hypertrophy model was created by using 50 μmol/L H2O2,and they were randomly divided into control group(group C),cardiac hypertrophy group(group H)and cardiac hypertrophy+PTE group(group H+PTE).After 48 hours of intervention,surface area of H9C2 cardiomyocytes was detected by using cellular immunofluorescence,protein p62 and p-mTOR expression were detected by using Western blot.Results Compared with group C,surface area of cardiomyocytes and expression of p62 significantly increased in group H,and expression of p-mTOR/mTOR significantly decreased in group H(P<0.05).Compared with group H,surface area of cardiomyocytes and expression of p62 significantly decreased in group H+PTE,and expression of p-mTOR/mTOR significantly increased in group H+PTE(P<0.05).Conclusion PTE alleviates H2O2-induced hypertrophy in H9C2 cardiomyocytes by regulating autophagy through inhibition of mTOR signaling pathway.
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