ZOPOMED-60结核分枝杆菌显微扫描仪检测抗酸杆菌效果
Evaluation of ZOPOMED-60 mycobacterium tuberculosis microscanner for detecting acid-fast bacilli in sputum smears
董捷 1唐柳生 1杨小兵 1黄丽玲2
作者信息
- 1. 广西壮族自治区胸科医院医学检验科,广西柳州 545005
- 2. 广西壮族自治区胸科医院科教科,广西柳州 545005
- 折叠
摘要
目的 评价ZOPOMED-60 结核分枝杆菌显微扫描仪在痰涂片查找抗酸杆菌的阅片效果.方法 收集 2023 年 5 月于广西壮族自治区胸科医院就诊患者的合格痰标本 203 份,分别采用萋-尼染色法和荧光染色法进行染色,通过人工镜检和ZOPOMED-60 结核分枝杆菌显微扫描仪进行抗酸杆菌检测,以分枝杆菌液体培养为标准,计算不同方法查找抗酸杆菌的敏感度、特异性、阳性预测值、阴性预测值.结果 荧光染色人工阅片和仪器智能阅片的阳性率分别为 12.3%和 18.7%,萋-尼染色人工阅片和仪器智能阅片的阳性率分别为 13.3%和 17.7%,4 种阅片检测结果差异无统计学意义(P>0.05).以分枝杆菌液体培养结果为标准,荧光染色人工阅片和仪器智能阅片、萋-尼染色人工阅片和仪器智能阅片检测抗酸杆菌的敏感度分别为 40.0%、45.0%、45.0%和 53.3%,特异性分别为 99.3%、92.3%、100.0%和 97.2%,阳性预测值分别为 96.0%、71.0%、100.0%和 88.9%,阴性预测值分别为 79.8%、80.0%、81.2%和 83.2%,一致性均较好.结论 两种染色方法应用ZOPOMED-60 结核分枝杆菌显微扫描仪检测抗酸杆菌的性能较好,值得推广应用.
Abstract
Objective To evaluate the effect of ZOPOMED-60 mycobacterium tuberculosis microscanner on detecting acid-fast bacilli in sputum smears.Methods A total of 203 qualified sputum specimens from patients treated in Chest Hospital of Guangxi Zhuang Autonomous Region in May 2023 were collected.The samples were stained by using Ziehl-Neelsen staining and fluorecent staining,respectively,and the acid-fast bacillus was detected by the manual microscopy and ZOPOMED-60 mycobacterium tuberculosis microscanner.The sensitivity,specificity,positive predictive value and negative predictive value of acid-fast bacillus were calculated using different methods based on liquid culture of mycobacterium.Results The positive rates of manual reading and instrument intelligent reading of fluorecent staining were 12.3%and 18.7%,respectively,and the positive rates of manual reading and instrument intelligent reading of Ziehl-Neelsen staining were 13.3%and 17.7%,respectively.There was no significant difference between the four reading results(P>0.05).Based on the results of liquid culture of mycobacterium,sensitivity of manual reading and instrument intelligent reading of fluorecent staining and manual reading and instrument intelligent reading of Ziehl-Neelsen staining were 40.0%,45.0%,45.0%and 53.3%,specificity were 99.3%,92.3%,100.0%and 97.2%,positive predictive values were 96.0%,71.0%,100.0%and 88.9%,and negative predictive values were 79.8%,80.0%,81.2%and 83.2%,respectively.The consistency was good.Conclusion The performance of ZOPOMED-60 mycobacterium tuberculosis microscanner in the detection of acid-fast bacilli by two staining methods is good,and it is worth popularizing.
关键词
显微扫描仪/抗酸杆菌/敏感度/特异性/一致性Key words
Microscanner/Acid-fast bacilli/Sensitivity/Specificity/Consistency引用本文复制引用
基金项目
广西壮族自治区医疗卫生适宜技术开发与推广应用项目(S2022042)
出版年
2024
国家科技期刊平台
NSTL
万方数据