Evaluation of ZOPOMED-60 mycobacterium tuberculosis microscanner for detecting acid-fast bacilli in sputum smears
Objective To evaluate the effect of ZOPOMED-60 mycobacterium tuberculosis microscanner on detecting acid-fast bacilli in sputum smears.Methods A total of 203 qualified sputum specimens from patients treated in Chest Hospital of Guangxi Zhuang Autonomous Region in May 2023 were collected.The samples were stained by using Ziehl-Neelsen staining and fluorecent staining,respectively,and the acid-fast bacillus was detected by the manual microscopy and ZOPOMED-60 mycobacterium tuberculosis microscanner.The sensitivity,specificity,positive predictive value and negative predictive value of acid-fast bacillus were calculated using different methods based on liquid culture of mycobacterium.Results The positive rates of manual reading and instrument intelligent reading of fluorecent staining were 12.3%and 18.7%,respectively,and the positive rates of manual reading and instrument intelligent reading of Ziehl-Neelsen staining were 13.3%and 17.7%,respectively.There was no significant difference between the four reading results(P>0.05).Based on the results of liquid culture of mycobacterium,sensitivity of manual reading and instrument intelligent reading of fluorecent staining and manual reading and instrument intelligent reading of Ziehl-Neelsen staining were 40.0%,45.0%,45.0%and 53.3%,specificity were 99.3%,92.3%,100.0%and 97.2%,positive predictive values were 96.0%,71.0%,100.0%and 88.9%,and negative predictive values were 79.8%,80.0%,81.2%and 83.2%,respectively.The consistency was good.Conclusion The performance of ZOPOMED-60 mycobacterium tuberculosis microscanner in the detection of acid-fast bacilli by two staining methods is good,and it is worth popularizing.
国家科技期刊平台
NSTL
万方数据
