Effects of arsenic trioxide on DNA methyltransferases expression of nasopharyngeal carcinoma cell lines
Objective To investigate the effect of arsenic trioxide(As2O3)on the expression levels of DNA methyltransferases(DNMT)(DNMT1,DNMT3A and DNMT3B)in nasopharyngeal carcinoma cell lines.Methods Incubation of 8 μmol/L As2O3 with nasopharyngeal carcinoma cell line C666-1,CNE1,CNE2 and nasopharyngeal epithelial immortal cell line NP69 cells for 48h,cell counting kit-8 was measured the proliferation of nasopharyngeal carcinoma cells.Moreover,quantitative reverse transcriptase-mediated polymerase chain reaction and Western blot were used to determine the levels of DNMT mRNA and protein expression in nasopharyngeal carcinoma cells and NP69 cells before and after As2O3 treatment.Results Compared with nasopharyngeal carcinoma cells,the expression of DNMT1 and DNMT3A in NP69 cells were the lowest.After being incabation with the As2O3,NPC cell viability decreased with obvious morphological changes.It is observed that levels of DNMT expressed in various cells were not completely consistent,with C666-1 cells showing significantly lower expression levels for all three DNMT.However,DNMT1 and DNMT3B were downregulated in CNE1 and CNE2 cells,DNMT3A expression was significantly upregulated in CNE1 cells.Conclusion It is suggested that As2O3 has potential therapeutic value for nasopharyngeal carcinoma through which could inhibite the expression of DNMT.
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