Clinical study on the effects of snake bites on neutrophil traps(NETs)and inflammatory damage
Objective To investigate the changes in neutrophils and their extracellular trap(NETs)markers,including neutrophil elastase(NE)and cell-free DNA(cf-DNA),after Gloydius brevicaudus biten and their correlation with inflammatory and tissue damage markers.Methods A total of 36 patients biten by Gloydius brevicaudus and 20 healthy individuals were included in the study from September 2021 to December 2022 at Hangzhou Hospital of Traditional Chinese Medicine.Neutrophil count,lymphocyte count,neutrophil-to-lymphocyte ratio(NLR),C-reactive protein(CRP),serum amyloid A(SAA),aspartate aminotransferase(AST),lactate dehydrogenase(LDH),creatine kinase(CK),creatine kinase-MB(CKMB),cf-DNA,and NE levels were compared between the two groups.Pearson correlation coefficients were calculated,and a heatmap and boxplot were generated using Python software to analyze the correlation between neutrophil count and NETs markers,inflammatory markers,and tissue damage markers.Results After Gloydius brevicaudus biten,Neutrophil count,NE,cf-DNA,CRP,SAA,NLR,and AST were elevated,while lymphocyte count was decreased.There was a general positive correlation between Neutrophil count and NE,as well as cf-DNA/NETs serum levels.However,there was no significant correlation between Neutrophil count and inflammatory markers CRP and SAA.CRP was strongly positively correlated with SAA,and CKMB was strongly positively correlated with LDH.CK was highly positively correlated with NE and SAA was highly positively correlated with NE.Lymphocyte count had a strong negative correlation with NLR.Conclusion Gloydius brevicaudus biten may result in an increase in NETs levels in neutrophils,promoting inflammation exacerbation and tissue damage.
Gloydius brevicaudus bitesNeutrophilsNeutrophil extracellular trapSerum amyloid A
万方数据
维普
