Effect of lncRNA SNHG 15 in regulating miR-95-3p on the proliferation and apoptosis of breast cancer cells
Objective To explore the effect of long non-coding RNA(long non-coding RNA,lncRNA)small RNA host gene 15(small nucleolar RNA host gene 15,SNHG 15)regulating microRNA 95-3p(miR-95-3p)on breast cancer cell proliferation and apoptosis.Methods Breast cancer cells were divided into control group,knockdown group,interference group and co-transfection group.Human breast cancer cell line MDA-MB-231 was cultured in vitro.Measure the relative expression levels of SNHG 15 mRNA and miR-95-3p mRNA;Methylthiazolyldiphenyl-tetrazolium bromide(MTT)was used to detect the proliferation ability of breast cancer cells;The apoptosis of breast cancer cells was detected by flow cytometry;The migration ability of breast cancer cells was detected by scratch test;Transwell invasion test was used to measure the number of breast cancer cells invaded;Western blot was used to determine the relative expression levels of caspase-3,B-cell lymphoma-2(Bcl-2),and Bcl-2 associated X protein(Bax).Results Compared with control group,the relative expression level of SNHG 15 mRNA and miR-95-3p mRNA,proliferation ability,proliferation rate,migration ability,number of invasion,Bcl-2 protein expression were decreased,but the apoptosis status,apoptosis rate,caspase-3 and Bax expression were increased in knockdown group(P＜0.05).Compared with knockdown group and interference group,the relative expression level of SNHG 15 mRNA and miR-95-3p mRNA,proliferation capacity,proliferation rate,migration capacity,number of invasion,Bcl-2 protein expression were increased,but apoptosis status,apoptosis rate,caspase-3 and Bax expression were decreased in co-transfection group(P＜0.05).Compared with interference group,the relative expression level of SNHG 15 mRNA and miR-95-3p mRNA,proliferation ability,proliferation rate,migration ability,number of invasion,Bcl-2 protein expression were increased,but apoptosis status,apoptosis rate,caspase-3 and Bax expression were decreased in co-transfection group(P＜0.05).Conclusion lncRNA SNHG 15 can affect the proliferation and apoptosis of breast cancer cells by regulating miR-95-3p expression,and is positively associated with the occurrence and development of breast cancer cells.
Long non-coding nucleolar small RNA host gene 15Long non-coding RNAmicroRNA 95-3pBreast cancerProliferationApoptosi
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