Analysis of mitochondrial DNA content and site 10398 in healthy and cervical cancer populations
Objective To explore the clinical significance of detecting mitochondrial DNA(mtDNA)content and 10398 site mutation in serum.Methods A tatol of 314 healthy women who underwent physical examinations at the Yichang Second People's Hospital were collected from January to December 2020 as health examination group,and collect 82 cervical cancer patients who received medical treatment at the Second People's Hospital of Yichang City from January 2020 to December 2023 as cervical cancer group.Detect the mtDNA content and 10398 locus genotype of serum samples from two groups of participants,and compare the differences in indicators between two groups of participants.Results The mtDNA content of cervical cancer patients was significantly higher than that of healthy examination group(P＜0.05);There was no statistically significant difference in genotype at locus 10398 between two groups of participants(P＞0.05).The mtDNA content of women aged≥45 in health examination group was higher,and the difference was statistically significant(P＜0.05);There was no statistically significant difference in the distribution of mtDNA at different 10398 loci(P＞0.05).Patients aged ≥45 in cervical cancer group have higher mtDNA content,and the difference is statistically significant(P＜0.05);There was no statistically significant difference in mtDNA content among patients with different clinical stages and pathological types(P＞0.05),and there was no statistically significant difference in mtDNA content at different 10398 loci(P＞0.05).Conclusion The detection of mtDNA content has certain significance for both healthy physical examination population and cervical cancer population.The mtDNA content in both groups is related to age and increases with age.The mtDNA content in cervical cancer population is significantly higher than that in normal physical examination population.The detection of mtDNA at 10398 site in serum has little significance for both groups.The mtDNA content and 10398 site distribution in cervical cancer population are not related to pathology and stage.The detection of mtDNA content can be considered as a serum biomarker for the diagnosis of cervical cancer.
Mitochondrial DNAThe mutation of site 10398Cervical cancerHealthy people
万方数据
维普
