Studies on preparation and properties of brain-targeted dual-anesthetics nanocomposites
Objective To construct a lactoferrin-modified brain-targeted propofol-sodium hydroxybutyrate dual-anesthetics starch nanocomposite(Lf-SNPs)and evaluate its brain targeting and optimized anesthetic property.Methods In this study,natural starch was selected as the nanomaterial,lactoferrin as the brain targeting protein,and Lf-SNPs was prepared by inverted phase emulsion method.The dual-loading and brain-targeted delivery of propofol and sodium hydroxybutyrate were achieved by hydrophobic and electrostatic forces,respectively.Lf-SNPs'particle size,potential,distribution in vivo were measured.Twenty C57BL/6 mice were randomly divided into two groups:Control group(same concentration of mixed anesthetics)and Lf-SNPs group,with 10 mice in each group.The duration of anesthetic efficacy was recorded after caudal vein administration.Results The Lf-SNPs had a particle size of(330.6±22.6)nm and average potential of(-23.4±0.5)mV,respectively.The distribution of Lf-SNPs in the brain was visible 30s after intravenous administration,the reaching peak at 3 min,and sustainable distributing for 30 min.The duration of anesthetic efficacy of single bolus infusion was(38.7±5.9)min,which was significantly longer than that of the same concentration of control group(P＜0.05).Conclusion Lf-SNPs has the dual-loading capacity of propofol and sodium hydroxybutyrate,which can realize the targeted delivery in brain and enhance the anesthetic effect.
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