Causal association between gut microbiota and stroke:A two-sample Mendelian randomization analysis
Objective To investigate causal relationship between gut microbiota and stroke by using Mendelian randomization(MR).Methods The gut microbiota genome wide association study(GWAS)data of MiBioGen study in public database(n=13 266)and stroke GWAS data of IEU Open GWAS were used.Independent genetic loci significantly correlated with the relative abundance of gut microbiota at genus level were used for instrumental variables(Ⅳs)according to preset thresholds.The inverse variance weighted method was used to analyse,results were evaluted according to odds ratio(OR)of effect index and 95％confidence interval(CI).The stability and reliability of the results were verified by leave one out,heterogeneity test and horizontal gene pleiotropy test.Results The rise of abundance of LachnospiraceaeFCS020group genera,Lachnospiraceae NK4A136group genera,RuminococcaceaeUCG004 genera and Intestinimonas genera,could reduce the risk of stroke,the OR(95％CI)were 0.914(0.845-0.988),0.909(0.838-0.985),0.919(0.849-0.995),0.924(0.866-0.987),respectively.The increase of abundance of Allisonella genera,Gordonibacter genera,Paraprevotella genera and Streptococcus genera can increase the risk of stroke,and the OR(95％CI)were 1.103(1.039-1.171),1.056(1.009-1.105),1.078(1.013-1.147),1.110(1.020-1.209),respectively.Leave one out showed that the results were stable,there were no IVs that had a strong influence on the results,and the influence of heterogeneity and horizontal gene pleiotropy on the estimation of causal effect could be eliminated.Conclusion The levels of Lachnospiraceae FCS020group genera,LachnospiraceaeNK4A136group genera,RuminococcaceaeUCG004 genera and Intestinimonas genera protect factors of brain stroke,with the increase of bacterial abundance,the incidence of stroke may be reduced.On the contrary,the levels of Allisonella genera,Gordonibacter genera,Paraprevotella genera and Streptococcus genera are positively correlated with stroke occurrence.Probiotics in the treatment of stroke may become the trend of future clinical or mechanism research.
万方数据
维普
