Study on the clinical value of miR-145 combined with TGF-β1 detection in non-small cell lung cancer
Objective To detect the expression of microRNA 145(miR-145)and transforming growth factor β1(TGF-β1 )in non-small cell lung cancer(NSCLC)and study their clinical value.Methods 92 NSCLC patients(NSCLC group)and 45 patients with benign lung diseases(control group)diagnosed and treated from January 2020 to January 2021 in Hangzhou Hospital of Traditional Chinese Medicine were selected as the research subjects.The expression differences of miR-145 and TGF-β1 between two groups of research subjects were detected and compared.Receiver operating characteristic(ROC)curve was used to analyze the sensitivity and specificity of miR-145 combined with TGF-β1 in predicting poor prognosis of NSCLC.Multivariate Cox regression analysis was used for risk factors of prognosis in NSCLC patients.Kaplan-Meier model was used to analyze the relationship between miR-145,TGF-β1 and survival time.Results Compared with the adjacent normal tissue in NSCLC group and the biopsy tissue in control group,the expression of miR-145 in the tumor tissue of NSCLC group was significantly reduced(P＜0.001),and the expression of TGF-β1 was significantly increased(P＜0.001).The expression of miR-145 in NSCLC patients with T stage T3-T4,regional lymph nodes N2+N3,distant metastasis and TNM stage Ⅲ-Ⅳ is lower than that in T stage T1-T2,regional lymph nodes N0+N1,no distant metastasis and TNM stage Ⅰ-Ⅱ.TGF-β1 was higher than that in patients with T stage T1-T2,regional lymph nodes N0+N1,no distant metastasis and TNM stage Ⅰ-Ⅱ,and the difference was statistically significant(P＜0.001).The sensitivity and specificity of miR-145 combined with TGF-β1 in predicting poor prognosis in NSCLC patients was higher than that of miR-145,TGF-β1,T stage,regional lymph nodes,distant metastasis and TNM stage(P＜0.05).In NSCLC group,the median survival time of patients with miR-145 ≤0.61 and TGF-β1 ≥0.85 was significantly lower than that of other patients(miR-145＞0.61 or TGF-β1＜0.85)[median survival time(29.37±5.51)months vs.(34.34±6.09)months,Log Rank=16.478,P＜0.001].Conclusion The expression of miR-145 is significantly reduced and TGF-β1 is significantly increased in NSCLC patients,which has certain clinical value in the evaluation of NSCLC condition and prognosis.Combined detection of miR-145 and TGF-β1 can significantly improve the sensitivity and specificity in predicting poor prognosis of NSCLC.
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