Effect of SYT7 regulation of Hedgehog/Gli signaling pathway on the progression of glioblastoma
Objective To investigate the effect of synaptotagmin-7(SYT7)on the progression of glioblastoma by regulating Hedgehog/Gli signaling pathway.Methods The expression of SYT7 in U87 cell line was analyzed by quantitative reverse transcriptase-mediated polymerase chain reaction(qRT-PCR)and transfection with short hairpin RNA(shRNA)lentiviral vector.Western blotting and qRT-PCR were used to analyze the knockdown efficiency of SYT7 shRNA in U87 cells.Cell counting,cell cycle analysis,and animal studies confirm the role in glioblastoma.Results qRT-PCR analysis showed that the expression of SYT7 in U87 cell line was higher than human normal glial cell line.Western blotting and qRT-PCR analysis showed low knockdown efficiency of SYT7 shRNA in U87 cells.Cell counts showed that the lentiviral vector expressing SYT7 shRNA down-regulated SYT7 and inhibited the growth of U87 cells.Flow cytometry confirmed cell cycle block,gene enrichment analysis and Western blotting revealed that SYT7 was associated with Hedgehog/Gli signaling pathway by promoting p53 phosphorylation.It was further demonstrated in glioblastoma by animal experiments.Conclusion The results of this study suggest that SYT7 promotes glioblastoma growth by regulating the Hedgehog/Gli signaling pathway,so SYT7 may be a potential therapeutic target for glioma.
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