目的 探讨肿瘤蛋白p53诱导的核蛋白2(tumor protein p53 inducible nuclear protein 2,TP53INP2)和溶质载体有机阴离子转运蛋白家族成员2A1(solute carrier organic anion transporter family member 2A1,SLCO2A1)在分化型甲状腺癌(differentiated thyroid carcinoma,DTC)中的表达及其与临床病理特征和预后的关系.方法 选取2024年3月至5月于南京医科大学第三附属医院行甲状腺癌根治术的DTC患者80例,比较TP53INP2和SLCO2A1在DTC患者的癌组织、癌旁组织及淋巴结转移组织中的表达,分析其与DTC患者临床病理特征之间的关系.从癌症基因组图谱(The Cancer Genome Atlas,TCGA)数据库下载正常人甲状腺和DTC组织的表达矩阵、临床病理参数及预后资料,使用R软件进行基因集变异分析(gene set variation analysis,GSVA),分析GSVA评分与DTC患者临床病理特征及预后之间的关系.采用Kaplan-Meier法分析基因集GSVA评分与DTC患者无进展生存(progress free survive,PFS)之间的关系.采用Cox单因素及多因素分析探讨影响DTC患者预后的因素.结果 DTC患者癌组织及淋巴结转移组织中的TP53INP2和SLCO2A1表达显著降低(P<0.05).GSVA评分与肿瘤侵袭、淋巴结转移、远处转移和病理类型显著相关(P<0.05).GSVA评分低的DTC患者的PFS率显著低于GSVA评分高者(P=0.002).Cox多因素分析结果显示肿瘤侵袭及GSVA评分低均是导致DTC患者预后不良的独立影响因素(P<0.05).结论 TP53INP2和SLCO2A1在DTC组织中表达降低,其表达降低与肿瘤侵袭、淋巴结转移、远处转移及不良预后有关,或可成为DTC患者预后判断的潜在靶点.
Relationship between TP53INP2 and SLCO2A1 with clinicopathological features and prognosis of differentiated thyroid carcinoma
Objective To investigate the expression of tumor protein p53 inducible nuclear protein 2 (TP53INP2) and solute carrier organic anion transporter family member 2A1 (SLCO2A1) in differentiated thyroid carcinoma (DTC) and their relationship with clinicopathological features and prognosis. Methods A total of 80 DTC patients who underwent radical thyroidectomy in the Third Affiliated Hospital of Nanjing Medical University from March to May 2024 were selected. The expression of TP53INP2 and SLCO2A1 in cancer tissue,paracancer tissue and lymph node metastasis tissue of DTC patients were compared,and the relationship between TP53INP2 and SLCO2A1 with clinicopathologic features of DTC patients were analyzed. Expression matrix,clinicopathological parameters and prognostic data of normal thyroid and DTC tissues were downloaded from The Cancer Genome Atlas (TCGA) database,and gene set variation analysis (GSVA) was performed using R software. The relationship between GSVA score and clinicopathological features and prognosis of DTC patients was analyzed. Kaplan-Meier method was used to analyze the relationship between gene set GSVA score and progress free survival (PFS) in DTC patients. Cox univariate and multivariate analysis was used to investigate the factors affecting the prognosis of DTC patients. Results The expressions of TP53INP2 and SLCO2A1 in cancer tissues and lymph node metastasis tissues of DTC patients were significantly decreased (P<0.05). GSVA score was significantly correlated with tumor invasion,lymph node metastasis,distant metastasis and pathological type (P<0.05). The PFS rate of DTC patients with low GSVA score was significantly lower than that of those with high GSVA score (P<0.05). Cox multifactor analysis showed that tumor invasion and low GSVA score were independent factors leading to poor prognosis in DTC patients (P<0.05). Conclusion The expression of TP53INP2 and SLCO2A1 is decreased in DTC tissues,which is associated with tumor invasion,lymph node metastasis,distant metastasis and poor prognosis,and may be a potential target for prognostic judgment in DTC patients.
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