Evaluation of mouse 2K1C renal artery stenosis model by high-frequency ultrasound in experimental small animals
Objective To investigate the method of evaluating renal artery stenosis (RAS) model in mice by high-frequency ultrasound in experimental small animals. Methods C57B1/6J male mice were selected and left renal artery was clamped with silver clip to prepare RAS model (RAS group,n=5),while left renal artery was not clamped in sham operation group (n=5). Systolic blood pressure was measured 3 days after surgery. Four weeks after surgery,the left ventricular movement curve was recorded at the level of parasternal left ventricular brachyaxis papillary muscle by high-frequency ultrasound of experimental small animals,mice left ventricular anterior wall and posterior wall thickness were measured,left ventricular mass,ejection fraction and left ventricular short axis reduced rate were calculate. The renal morphological parameters (kidney length,width,thickness and cortical thickness),the vascular wall thickness and resistance index of renal artery were measured. The right renal artery distensibility and global radial strain of the vessel wall were evaluated by two-dimensional dot tracking technique. Renal pathology was observed 4 weeks after surgery. Results Compared with sham operation group,systolic blood pressure,left ventricular anterior wall and posterior wall thickness and left ventricular mass were significantly increased in RAS group (P<0.05). Compared with sham operation group,the length,width,thickness and cortical thickness of left kidney in RAS group were significantly reduced (P<0.05). In the right kidney,the compensation increased,the thickness of the renal cortex increased,vascular wall of right renal artery was significantly thickened,distensibility and global radial strain of right renal artery were significantly reduced (P<0.05). Conclusion The application of high-frequency ultrasound in experimental small animals can noninvasively detect the damage of the heart,kidney and renal vessels in RAS model,and analyze the biomechanical indexes such as renal artery strain,providing a new method for the detection of mouse RAS model.
Two kidneys and one clipHigh-frequency ultrasound in experimental small animalsTwo-dimensional speckle tracking techniqueNoninvasive detectionMice
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