miR-625-5p targets LASP1 to inhibit the proliferation and migration of colorectal cancer cells and promote apoptosis
Objective To investigate whether miR-625-5p can target LIM and SH3 protein 1(LASP1)to affect the proliferation,migration and apoptosis of colorectal cancer cells.Methods Colorectal cancer(CRC)cells were transfected into control group,NC group,miR-625-5p mimic group,miR-625-5p inhibitor group and miR-625-5p mimic+LASPl group.Quantitative polymerase chain reaction(qPCR)assay was performed to detect mRNA expression of LASP1 and miR-625-5p in each group,dual-luciferase reporter gene was used to detect gene targeting binding relationship,cell activity was detected by cell counting kit(CCK)-8,cell cloning ability was detected by cell plate cloning,cell apoptosis was detected by flow cytometry,and cell migration and invasion were detected by Transwell assay.Cell proliferation was measured by EDU assay,and expression of LASP1 and invasion and metastasis related proteins neural cadherin(N-cadherin)and epithelial cadherin(E-cadherin)were detected by Western blot.Results qPCR experiment proved that cell transfection was successful.The dual luciferase reporter gene detected that miR-625-5p could target LASP1.Compared with NC group,cell activity,cell cloning,migration,invasion,proliferation and protein expression levels of LASP1 and N-cadherin in miR-625-5p mimic group were significantly decreased(P＜0.05).Apoptosis rate and E-cadherin protein expression level were significantly increased(P＜0.05),but expression of above indicators was completely opposite in miR-625-5p inhibitor group.Compared with miR-625-5p mimic+LASP1 group,cell activity,cell cloning,migration,invasion,proliferation and LASP1 protein expression level were significantly increased(P＜0.05),and cell apoptosis rate was significantly decreased(P＜0.01).Conclusion miR-625-5p can target LASP1 to inhibit the proliferation and migration of CRC and promote apoptosis.
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