注射用血栓通(冻干)透过血脑屏障研究
Research on Xueshuantong Injection through blood brain barrier
王旭梅 1赵莼 1王少峡 1林晓燕 2刘晓雷1
作者信息
- 1. 天津中医药大学中医药研究院,天津市中药药理学重点实验室,教育部方剂学重点实验室,天津300193
- 2. 瀚盟生物技术(天津)有限公司,天津300457
- 折叠
摘要
目的 研究大鼠脑缺血再灌注后,血栓通主要成分人参皂苷Rb1、Rg1、Re、Rd及三七皂苷R1在脑内的分布状况.方法 采用超高效液相色谱质谱联用法(UPLC-MS/MS)测定给予不同浓度的血栓通后,不同时间点血栓通主要成分在正常脑组织和缺血脑组织中的含量.结果 结果表明,血栓通活性成分在脑缺血大鼠脑中的分布远高于正常大鼠,并且其在脑内的含量随再灌注时间的延长降低.结论 脑缺血再灌注后,血脑屏障开放,注射用血栓通(冻干)可以透过血脑屏障发挥作用.
Abstract
Objective To study the intracephalic distribution of ginsenosides Rb1,Rg1,Re,Rd and notoginsenoside R1 from Xueshuantong Injection (lyophilized powder) after ischemia-reperfusion.Methods Using ultra performance liquid chromatography tandem massspectrometry (UPLC-MS/MS),the contents of active ingredients in Xueshuantong Injection in normal brain tissue and ischemic tissue at different drug concentration and time points were measured.Results The distribution of active ingredients of Xueshuantong Injection in cerebral ischemia brain was much higher than that of normal ones,and the content of active ingredients in brain reduces with reperfusion prolonged.Conclusion Xueshuantong Injection plays its role because blood brain barriar opens after ischemia-reper fusion.
关键词
注射用血栓通/血脑屏障/缺血再灌注/UPLC-MS/MS/人参皂苷Rb1/人参皂苷Rg1/人参皂苷Re/人参皂苷Rd/三七皂苷R1Key words
Xueshuantong Injection/blood brain barrier/ischemia-reperfusion/UPLC-MS/MS/ginsenoside Rb1/ginsenoside Rg1/ginsenoside Re/ginsenoside Rd/notoginsenoside R1引用本文复制引用
基金项目
国家重大新药创制(2012ZX09101201-004)
高等学校博士学科点专项科研基金(20101210110002)
天津市应用基础与前沿技术研究计划(14JCYBJC28900)
广西科学研究与技术开发计划(14125008-2-5)
出版年
2015
NETL
NSTL
维普
万方数据