Preparation of honokiol liposomes and their in vitro and in vivo suppression on breast cancer
Objective To prepare honokiol liposomes (HK Liposomes) and explore their inhibition of breast cancer cells (4T1) in vitro and in vivo.Method The HK Liposomes were prepared by ethanol injection method using egg yolk lecithin,cholesterol,mPEG2000-DSPE2000 and honokiol (3∶1∶1∶1,weight ratio) in formulation.The size of HK liposomes was determined by Zetasizer nano ZS.The morphology of HK Liposomes was observed by transmission electron microscope.MTT assay was used to evaluate the cytotoxic activity of HK liposomes against 4T1 murine breast cancer cell line with free HK as a control.The in vivo antitumor effect of the resultant HK Liposomes (ip administration) was evaluated on 4Tl-bearing mice at different dose using PTX injection as a positive control.Results The HK liposomes were (113.8 ± 0.209) nm in average particle size with smooth surface and spherical shape.The polydispersity index (PDI) value was (0.209 ± 0.005) and zeta potential was (-30.7 ± 2.4) mV.Honokiol liposomes showed significant higher cytotoxicity than free HK against 4T1 cells (IC50 value 12.52 μg/mL vs 43.74 μg/mL,P ＜ 0.01).On 4T1 tumor-bearing mice models,HK liposomes demonstrated a dose-dependent tumor suppression effect with inhibitory rate being 37.83％,60.95％ and 85.19％ respectively for low (20 mg/kg),middle (40 mg/kg),and high dose (60 mg/kg).Conclusion In contrast,HK liposomes were successfully prepared with high encapsulation efficiency (94％),small particle size (113.8 nm) and demonstrated enhanced in vitro and in vivo anti-tumor efficacy in comparison with free HK.
NETL
NSTL
万方数据
维普
