Establishment of fingerprint,component identification,and in vitro antioxidant activity of Camellia nitidissima
Objective To establish a HPLC fingerprint of Camellia nitidissima Chi,analyze its chemical components,and study its antioxidant capacity.Methods Extraction of ten batches of Camellia nitidissima Chi were made by refluxing with 70％ ethanol solution.Agilent Eclipse Plus C18(250 mm × 4.6 mm,5 μm)Chromatographic column were used,gradient elution,to establish a fingerprint of Camellia nitidissima Chi,analysis of its chemical components using HPLC-MS,and evaluation of the antioxidant capacity of Camellia nitidissima Chi using DPPH and ABTS methods.Results The HPLC chromatogram of Camellia nitidissima Chi was established,and 11 peaks were calibrated.The similarity was all above 0.98 through calculation of TCM chromatographic fingerprint similarity evaluation software(2012 edition).Meanwhile,HPLC-MS analysis showed that the components of Camellia nitidissima Chi were mostly flavonoids,and the antioxidant capacity of Camellia nitidissima Chi was tested.The results showed that it had good antioxidant capacity to DPPPH free radicals and ABTS free radicals in vitro,and showed a certain concentration dependence.Conclusion We had established a fingerprint of Camea nitidissima Chi,which was rich in flavonoids and had good in vitro antioxidant effects.
NETL
NSTL
万方数据
维普
