Comparative study on mechanism of Schisandra sphenanthera and Schisandra chinensis in treatment of Alzheimer's disease based on network pharmacology
Objective To explore the similarities and differences between the mechanism of Schisandra sphenanthera Rehd.et Wils.and Schisandra chinensis(Turcz.)Baill.in treatment of Alzheimer's disease(AD)based on network pharmacology and molecular docking techniques.Methods The active components of S.sphenanthera and S.chinensis were screened from the Traditional Chinese Medicine System Pharmacology Database(TCMSP)and published literature,access to drug and disease targets using the Swiss Target Prediction,OMIM,GeneCards databases,and draw the network diagram of"drug-active component-intersection target"by Cytoscape 3.9.1.Venny 2.1 was used to screen common and unique targets.Protein-protein interaction(PPI)network was constructed by.String database and Cytoscape 3.9.1.CytoNCA tool of Cytoscape 3.9.1 was used to screen key targets by target topology analysis.The Metascape platform performs GO functional and KEGG pathway enrichment analysis for common and unique targets.Finally,AutoDock Vina was used to verify the molecular docking of core active components and key targets.Results There were 15 active components of S.sphenanthera,13 active components of S.chinensis,there are 235 common targets for treating AD,137 unique targets of S.sphenanthera for treating AD,and 69 unique targets of S.chinensis for treating AD.The PI3K-Akt signaling pathway,neuroactive ligand-receptor interaction,calcium signaling pathway and MAPK signaling pathway were common signal pathways of S.sphenanthera and S.chinensis in treating AD.Th17 cell differentiation and PPAR signaling pathway were the unique regulatory signal pathways of S.sphenanthera in treating AD.The cGMP-PKG signaling pathway and chemokine signaling pathway were the unique regulatory signal pathways of S.chinensis in treating AD.Molecular docking results showed that S.sphenanthera core active components schisandrin,schisandrin A,schisantherin A,neokadsuranin and neokadsuranic acid B had good binding activity with key targets CASP3,ESR1 and HIF1A.S.chinensis core active components schisandrin,schisandrin A,schisantherin A,deoxyharringtonine and gomisin A had good binding activity with key targets ADRB2,SLC6A4 and ADRBK1.Conclusion Due to the different origins of S.sphenanthera and S.chinensis,the active components and therapeutic AD targets are both the similarities and differences.S.sphenanthera and S.chinensis can play a role in the treatment of AD through their common and respective unique targets.
Schisandra sphenanthera Rehd.et Wils.Schisandra chinensis(Turcz.)Baill.Alzheimer's diseasenetwork pharmacologyschisandrinschisandrin Aschisantherin A
NETL
NSTL
万方数据
维普
