Experimental study on content determination of three components and anti-inflammatory and hypolipidemic effects of Polygala fallax
Objective To establish a method for the determination of three components in Polygala fallax alcohol extract(PFAE)to investigate the anti-inflammatory and hypolipidemic effects of PFAE.Methods The contents of three components(tenuifolin,swertiamarin and rutin)in PFAE were determined by HPLC.The inflammatory models such as xylene induced ear swelling in mice,acetic acid induced increased permeability of abdominal capillaries in mice,and carrageenan induced paw swelling inflammation in mice models were established to evaluate the pharmacological effects of high,medium and low doses(40,20,and 10 g·kg-1)PFAE on acute and chronic inflammation.The ear swelling model of mice with bilateral adrenalectomy induced by xylene and the paw swelling model of mice with bilateral adrenalectomy induced by carrageenan were established to determine whether its anti-inflammatory effect depends on the hypothalamic-pituitary-adrenal axis(HPAA)system.The contents of malondialdehyde(MDA)and nitric oxide(NO)and the activity of superoxide dismutase(SOD)in the inflammatory tissue of the paw were determined by kits.A high-fat rat model was established by feeding SD rats with high-fat feed,and the effects of high,medium,and low doses(40,20,and 10 g·kg-1)of PFAE on serum total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)levels in hyperlipidemic rats were investigated.Results The indexes of methodological investigation were in accordance with the regulations.The average recovery rates of tenuifolin,swertiamarin and rutin were 97.13％-101.82％,and the RSD values were less than 3.0％.The mass fractions of tenuifolin,swertiamarin and rutin in the extract were 121.71,84.02 and 52.53 μg·g-1,respectively.Compared with model group,except for the low dose group was not statistically significant,the high and middle dose groups of PFAE had significant inhibition on ear swelling in mice(P＜0.05,0.01).The high,medium and low dose groups of PFAE had obvious inhibition on the permeability of abdominal capillary in mice(P＜0.05,0.01),and the swelling of foot caused by carrageenan had significant inhibition(P＜0.05,0.01).Compared with model group,in the mouse model of bilateral adrenalectomy,the high and medium dose groups of PFAE could significantly inhibit the ear swelling of the model mice induced by xylene(P＜0.05,0.01),and can significantly inhibit carrageenan-induced paw swelling in the model mice(P＜0.05,0.01),the high,medium and low dose groups of PFAE could significantly reduce the levels of MDA and NO in the toes,and significantly increase the content of SOD in the toes(P＜0.05,0.01).After 28 days of high-fat diet,compared with the model group,high,medium,and low doses of PFAE significantly reduced the levels of TC,TG,and LDL-C in rat serum(P＜0.05).Conclusion The content determination method established in this study has strong specificity,good repeatability and high accuracy,which can be used for the content determination of tenuifolin,swertiamarin and rutin in PFAE.PFAE has anti-inflammatory activity,and its anti-inflammatory effect may not depend on the hypothalamic pituitary adrenal axis(HPAA)system.At the same time,the anti-inflammatory mechanism of PFAE is related to reducing MDA,NO and increasing SOD activity.PFAE has obvious hypolipidemic effect on experimental hyperlipidemia.
NETL
NSTL
万方数据
维普
