Analgesic activity and mechanism of Yuntongding Capsule based on zebrafish model and network pharmacology
Objective To explore the analgesic activity of Yuntongding Capsules(YTD)based on zebrafish model and analgesic mechanism of Yuntongding Capsules by network pharmacology.Methods Phorbol myristate acetate(PMA)was used to establish a zebrafic pain model,and aspirin was used as positive control.The movement trajectory of zebrafish was monitored with the behavior trajectory analysis system and the analgesic activity of YTD was evaluated by the movement states such as movement frequency,movement time,movement distance and movement speed.Network pharmacology and molecular docking techniques were used to predict the core components and potential analgesic mechanism of YTD.Selecting network pharmacology prediction components of falcarindiol(Fal)and aurantiamide acetate(Aur)as the experimental drugs to verify the network pharmacology prediction results.Results By comparing the exercise intensity and trajectory of zebrafish in the model group and each administration groups,YTD has better analgesic activity,and it is better than the analgesic effect of Ligusticum or Armillaria mellea alone.87 active components and 159 action targets of YTD analgesia were screened by network pharmacology.Core components such as Aur and core targets such as MAPK1 were obtained by protein-protein interaction network analysis and molecular docking was performed,showing that the core component targets had good binding activity.Data from the Fal and Aur groups suggest that favorable analgesia can be achieved by administering Fal or Aur alone.This is consistent with the results of network pharmacology.Conclusion YTD can alleviate the pain of zebrafish exposed to PMA and has obvious analgesic effect.Predicting its mechanism of action may be related to processes such as reducing nerve damage and inflammatory response,and promoting cell apoptosis.At the same time,Fal and Aur are the core components that exert analgesic effects,providing a foundation for the research and development of new analgesic drugs in the future.
NETL
NSTL
万方数据
