Expression and distribution of asialoglycoprotein receptor(ASGPR)in N-dinitroethylamine-induced hepatocellular carcinoma model and orthotopic transplantation tumor mode in rats
Objective To determine the role of asialoglycoprotein receptor(ASGPR)in N-dinitroethylamine(DEN)-induced hepatocellular carcinoma model in rats(DEN-HCC-Rat)and orthotopic transplantation tumor mode in rats(OTT-HCC-Rat).Methods DEN-HCC-Rat modeling:SD rats in the model group were ig given a 0.25％ DEN aqueous solution of 20 mg·kg-1,once a week,with 0.025％ DEN aqueous solution for animal consumption;The control group received 0.9％sodium chloride solution once a week,sterilized water for animal consumption,and samples were taken at weeks 4,10,18,and 22 of modeling.OTT-HCC-Rat modeling:SD rats in the model group were injected with N1-S1 cells into the liver lobes,while rats in the sham surgery group were anesthetized and treated with minimally invasive suturing.Samples were taken 14 days after injection.HE staining was used to observe liver tissue lesions.Immunohistochemistry,immunofluorescence,Western blotting and real-time fluorescent quantitative PCR(qRT-PCR)were used to detect the expression and distribution in the liver tissue of two orthotopic liver cancer rat models and normal rats.Results In DEN-HCC-Rat,immunohistochemistry,immunofluorescence,Western blotting,and qRT-PCR results all showed that compared with the control group,the ASGPR expression in the liver tissue of the model group rats was significantly upregulated(P＜0.05,0.01),and showed a time correlation.In OTT-HCC-Rat,immunohistochemistry results showed that the expression of ASGPR in the model group was significantly higher than that in the sham surgery group(P＜0.05).Immunofluorescence,Western blotting,and qRT-PCR results showed no significant difference in ASGPR between the model group and the sham surgery group.Conclusion The DEN-HCC-Rat model can better simulate the changes of tumor microenvironment and the expression of ASGPR in liver cancer is higher than that in normal rat liver.Therefore,ASGPR can be used to mediate the transport of liver targeted agents and improve the therapeutic effect of liver targeted drugs.
asialoglycoprotein receptorN-dinitroethylamineinduced hepatocellular carcinoma rat modelorthotopic transplantation tumor rat modetarget
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