Research on effect and mechanism of Ziheche lyophilized powder to improve sleep
Objective To study the sleep-improving effect and mechanism by of Ziheche lyophilized powder(ZLP).Methods To establish the rat insomnia model by intraperitoneal injection of chlorophenylalanine(PCPA).The rats were divided into control group,model group,and low,medium,and high concentration groups(0.09,0.18,and 0.36 g·kg-1)of ZLP.Ig once a day for seven consecutive days,with the control group and model group receiving an equal amount of deionized water.The effect of ZLP on improving sleep was evaluated through general observation,righting experiment,open field experiment,forced swimming experiment,and tail suspension experiment,and the Western blotting technique was used to detect the expression levels of GABA-T,GAD65,and GAT-1 proteins,and preliminarily explore the mechanism of ZLP in improving sleep.Results Compared with the model group,the body weight increase of rats in the low,medium,and high dose groups of ZLP was significantly increased(P＜0.01),medium and high doses of ZLP have the effect of shortening sleep latency(P＜0.05)and prolonging sleep time(P＜0.01),a high dose of ZLP prolonged the retention time of the central grid(P＜0.01),increased the number of times the central grid was pierced(P＜0.01),and increased the number of standing and modification times in rats(P＜0.01),indicating that high-dose ZLP can reduce anxiety in insomnia rats(P＜0.05)and enhance their spontaneous exploration activity ability(P＜0.01);high dose ZLP shortened the floating time(P＜0.05)and prolonged the struggling time of rats(P＜0.01),indicating that high-dose ZLP can alleviate the depressive state of insomnia rats.Western blotting results showed that compared with the model group,the expression level of GABA-T in the hypothalamic tissue of rats treated with ZLP decreased(P＜0.05),while the expression levels of GAD65 and GAT-1 increased(P＜0.05 and 0.01).Conclusion ZLP may improve sleep by regulating the expression levels of GABA-T,GAD65,and GAT-1 proteins.
Ziheche lyophilized powderimprove sleepWestern blottingGABA acid system accessmechanism of action
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