Study on mechanism of Coptis chinensis regulating MAPK/ERK pathway in microcirculation and Th1/Th2 cytokines in rats with oral ulcers
Objective To investigate the mechanism of Coptis chinensis Franch.mediated mitogen activated protein kinase(MAPK)/extracellular signal regulated kinase(ERK)on microcirculation and Th1/Th2 cell levels in rats with oral ulcers.Methods The rats were randomly divided into control group,model group,Coptis chinensis 20,40,80 mg·kg-1 group,and watermelon cream(200 mg·kg-1)group,with 10 rats in each group.Except for the control group,oral ulcer models were prepared using the burning method with 40％acetic acid.Each treatment group was coated and administered once every 2 days for 7 consecutive days.Blood samples were taken from the abdominal aorta to detect microcirculation indicators.Hematocrit(HCT)was measured using a microcapillary method,serum fibrinogen(FIB)levels were measured using a reagent kit method,and plasma viscosity was measured using a vertebral plate viscometer.Serum levels of inflammatory factors interleukin-6(IL-6),interleukin-8(IL-8),tumor necrosis factor-α(TNF-α)and serum levels of Th1,Th2 cell-related factors IFN-γ and IL-10 were detected by ELISA.The histopathological changes of ulcer mucosa were observed by HE staining.The expression of ERK1/2,p-ERK1/2,p38 MAPK and p-p38 MAPK in mucosal tissues was detected by Western blotting.Results Compared with the model group,the oral ulcer area of Coptis chinensis 20,40,80 mg·kg-1 group was significantly reduced(P＜0.05),and pathological changes such as mucosal epithelial cell lysis,inflammatory response,and tissue necrosis were significantly reduced,HCT,FIB,and plasma viscosity were significantly reduced(P＜0.05),the levels of inflammatory factors IL-6,IL-8,and TNF-α in serum were significantly reduced(P＜0.05).The level of IFN-γ and IFN-γ/IL-10 in serum significantly decreased,while the level of IL-10 significantly increased(P＜0.05).The expression of ERK1/2,p-ERK1/2,p38 MAPK,and p-p38 MAPK proteins was significantly reduced(P＜0.05),and the effect of Coptis chinensis 80 mg·kg-1 was the most significant.Conclusion Coptis chinensis can improve microcirculation in rats with oral ulcers,maintain a balanced state of Th1/Th2 cells in the body,which may be related to the inhibition of the MAPK/ERK pathway.
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