Pharmacological mechanism of YishenYangxin Anshen Tablets on learning and memory in stress rats
Objective To explore the pharmacological mechanism of Yishen Yangxin Anshen(YYA)Tablets by axial regulation of the hypothalamic-pituitary-adrenal pathway on the model of stress rats.Methods SPF grade SD rats were randomly divided into control group,model group,Eszolam tablets(ES,positive control,0.6 mgkg-1),AnshenBuNao Ye(AS,positive control,2 mL·kg-1),and YYA high,medium,and low dose(YYA 7.2,3.6,1.8 g·kg-1)groups.The model of stress rats was made by intraperitoneal injecting cyclophosphamide and caffeine and water environment.After successful modeling,ig administration was administered once a day for 28 consecutive days.Morris Water Maze was used to detect learning memory ability.HE staining was used to observe the pathological changes in the hippocampus.Immunohistochemistry and RT-PCR were applied to detect the expression of nerve growth factor(NGF)and vasoactive intestinal peptide(VIP)in the hippocampus.ELISA was used to detect the level of serum adrenocorticotropic hormone(ACTH),adrenocorticoid-releasing hormone(CRH),adrenal corticoid hormone(ACH),and surperoxide dismutase(SOD),5-hydroxytryptamine(5-HT)content in the brain tissue.Results Compared with the model group,the time for rats to cross the platform and explore in the target quadrant significantly increased in each treatment group,with the ES group and YYA medium and high dose groups being particularly significant(P＜0.05).The degree of neuronal damage in the hippocampus has significantly improved.The staining of NGF and VIP in the cytoplasm of hippocampal neurons was significantly enhanced,and the expression levels of NGF and VIP mRNA were significantly increased(P＜0.05,0.01).The SOD levels in the ES group,YYA medium and high-dose groups significantly increased(P＜0.01).The levels of ACTH and CRH in the serum of each treatment group were significantly reduced(P＜0.05,0.01),while the levels of ACH in the AS group and YYA high-dose group were significantly reduced(P＜0.05).The levels of 5-HT were significantly increased in the AS group,ES group,and YYA high-dose group(P＜0.05,0.01).Conclusion YYA can reduce the oxidative stress level of the brain tissue,reduce the hippocampal tissue damage,protect nerves,and enhance the learning and memory ability of the stressed rats by inhibiting the HPA pathway.
Yishen Yangxin Anshen Tabletschronic stresshypothalamic-pituitary-adrenal axishippocampuslearning and memory
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