Study on anti-aging effect of raffinose based on model of Caenorhabditis elegans
Objective To study the anti-aging activity of raffinose and to explore its anti-aging mechanism.Methods Raffinose(150,200,and 250 μmol·L-1)was added to Escherichia coli to incubate Caenorhabditis elegans,the body bending frequency,head and tail swinging frequency,and body length of the nematodes were observed under a microscope,and the survival time of the Caenorhabditis elegans under heat stress stimulation was observed.The effect of raffinose on the anti-aging ability of nematode was evaluated by lipofuscin,and the anti-aging mechanism was determined by DAF-16 nuclear localization and SOD-3 expression.Results In the body bending test,the three concentration groups of raffinose accelerated the body bending frequency of Caenorhabditis elegans campared with control group(P＜0.001).In the head-tail swing test,the raffinose of 200 μmol·L-1 concentration group accelerated the head-tail swing frequency of nematodes campared with control group(P＜0.01).In the body length test,the three concentration groups of raffinose had no toxic effect on the growth and development of nematode.In the heat stress test,the three concentration groups of raffinose prolonged the survival time of Caenorhabditis elegans under heat stress(P＜0.05).In the lipofuscin test,the three concentration groups of raffinose decreased the content of lipofuscin in Caenorhabditis elegans.In DAF-16 nuclear localization assay,raffinose at three concentrations promoted the expression of DAF-16 protein into the nucleus.In the determination of SOD-3 expression,the level of SOD-3 protein expression increased in raffinose 250 μmol·L-1 group,which together with DAF-16 nuclear localization assay showed that raffinose's anti-aging activity depended on IIS molecular signal pathway.Conclusion Raffinose can promote the activity of Caenorhabditis elegans,has no toxicity to its growth and development,and can delay the senescence of Caenorhabditis elegans,promote the expression of DAF-16 protein into nucleus and increase the expression level of SOD-3 protein.The anti-aging mechanism of raffinose depends on IIS molecular signal pathway.
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