Study on HPLC fingerprint and multi-index determination of Shenxian-Shengmai Oral Liquid
Objective To establish the characteristic fingerprint of Shenxian-Shengmai Oral Liquid and the quantitative method of multi-index components.Methods The Ultimate LP-C18(250 mm × 4.6 mm,5 μm)column was used with acetonitrile and 0.2％phosphoric acid aqueous solution as mobile phase gradient elution.The flow rate was 1.0 mL·min-1.The column temperature was 30 ℃,and the detection wavelength was 270 nm(0-16 min,24-38 min)and 210 nm(16-24 min,38-100 min).Ten batches of Shenxian-Shengmai Oral Liquid were analyzed by using traditional Chinese medicine chromatographic fingerprint similarity evaluation software(2012 edition),HPLC fingerprints were established,and the contents of 10 index components were determined.Results A total of 28 common peaks were calibrated,of which 12 common peaks were identified,and the similarity of the fingerprints between 10 batches of samples was above 0.934.The 10 components of quantitative analysis were danshensu,pseudoephedrine hydrochloride,psoralenoside,isopsoralenoside,rosmarinic acid,salvianolic acid B,psoralen,isopsoralen,epmedin C,icariin,respectively.The contents were 1.218-2.781 mg mL-1,0.333-0.702 mg mL-1,2.391-4.120 mg·mL-1,2.083-3.486 mg mL-1,0.151-0.203 mg mL-1,0.158-1.046 mg mL-1,0.189-0.286 mg mL-1,0.196-0.305 mg mL-1,0.231-0.403 mg·mL-1and 0.323-0.512 mg·mL-1,respectively.Conclusion The established fingerprint and multi-index component quantitative method are accurate and reliable,and can be used for comprehensive quality control of Shenxian-Shengmai Oral Liquid.
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