Quantitative control of multi-component HPLC and comprehensive quality evaluation of chemometrics in Rosae Laevigatae Fructus
Objective To comprehensive quality evaluation of Rosae Laevigatae Fructus(RLF)from different producing areas,and to provide basis for studying the geoherbalism of RLF.Methods The 16 batches of RLF samples from eight provinces were collected.The high performance liquid chromatography(HPLC)method was used to simultaneous determine contents of euscaphic acid,tormentic acid,ursolic acid,gallic acid,catechin,hesperidin,rutoside,quercetin,luteolin,kaempferol,apigenin and ellagic acid,and the multi-components quantitative control mode of RLF was established.The principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)were used to screen potential markers affecting the quality of RLF.The quality evaluation model of RLF was established by using entropy weight-technique for order preference by similarity to an ideal solution(EW-TOPSIS)method,and the overall quality was evaluated comprehensively.Results The 12 components showed good linear relationships in their own ranges.The average recovery were 96.94％-100.05％with the RSDs of 0.72％-1.56％.Ellagic acid,catechin,quercetin and gallic acid,may be the main potential markers affecting the quality of RLF.The results of EW-TOPSIS analysis showed that the quality evaluation closeness(Jb)of 16 batches of RLF were 0.146 2-0.756 2,The quality of RLF from different producing areas was very different.Conclusion The established HPLC method is convenient in operation and accurate in results,and can be used as a quantitative control method for multi-components of RLF.Chemometrics and EW-TOPSIS method are objective and comprehensive,and can be used for the overall quality evaluation of RLF.
NETL
NSTL
万方数据
