Simultaneous determination of 26 inorganic elements by inductively coupled plasma-mass spectrometry in Caenorhabditis elegans at different periods of time
Objective A method was established to simultaneously determine the content of 26 elements in C.elegans by Inductively Coupled Plasma Mass Spectrometry(ICP-MS),and to analyze the content changes of elements in different stages of C.elegans growth and development.Methods The nematodes were treated by wet digestion using 65％—68％HN03 as the digestion solution,and 26 elements(Al,As,B,Ba,Bi,Ca,Cd,Co,Cr,Cu,Fe,Ga,In,K,Mg,Mn,Mo,Na,Ni,Pb,Sc,Se,Te,V,Zn,and Zr)in the nematodes were determined simultaneously using ICP-MS.The eggs obtained after synchronization of nematodes were evenly divided into five groups and cultured to L1,L2,L3,L4,and Adult stages,respectively.The content of 26 elements was determined using the established ICP-MS method.Results The results showed that the linearity of the 26 elements was good in the range of 0-500 μg·L-1 with R2 ≥ 0.999 3,the instrumental limits of detection were 0.000 1-0.090 3 μg·L-1,the instrumental precision RSD was 0.4％—1.8％,the reproducibility RSD was 0.0％—2.5％,the stability RSD was 0.8％—4.6％,the average spiked recoveries were 80.8％—118.5％,and the RSD was 0.2％—1.5％.The L4 stage nematodes have the highest overall element content,followed by the L2 stage,with the L1 stage nematodes having the lowest overall element content.Conclusion The content of elements in nematodes during growth and development was determined by ICP-MS,which is a fast,sensitive,simple and efficient method suitable for analyzing the distribution of elements in nematodes,and provides a certain theoretical basis for the further study of the potential effects of a single element or a variety of elements on Caenorhabditis elegans.
Caenorhabditis elegansinductively coupled plasma mass spectrometryinorganic elementscorrelation analysis
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