Effects of Rheum palmatum-Salvia miltiorrhiza drug pairs on colonic mucus barrier function in chronic renal failure rats
Objective To study the effects of Rheum palmatum-Salvia miltiorrhiza drug pairs(RS)on colonic mucus barrier function in rats with chronic renal failure(CRF).Methods Seventy male Wistar rats were randomly divided into sham-operation group,model group,Niaoduqing Granule(2.5 g·kg-1)group,RS high and low dose groups(6,3 g crude drug·kg-1),with 14 rats in each group.Except the sham-operation group,the CRF models were replicated by 5/6 nephrectomy.After continuous ig administration for 12 weeks,the levels of serum creatinine(Scr),urea nitrogen(BUN),interleukin(IL)-1β,IL-6 and tumor necrosis factor(TNF)-αwere measured with blood collected from abdominal aorta.The remaining kidney and colon tissue were dissected,the changes of renal histology and fibrosis were observed by HE and Masson staining.The changes of mucous layer thickness and goblet cell number in colon were observed by AB-PAS staining.The positive expression of Muc2 protein in colon tissues of rats was observed by immunohistochemistry.The expressions of Muc2 mRNA and protein in colon tissues was detected by real-time fluorescent quantitative PCR(qRT-PCR)and Western blotting.Results Compared with sham-operation group,the levels of serum Scr,BUN,IL-1β,IL-6 and TNF-α of rats in model group were significantly increased(P＜0.01),glomerular sclerosis,renal tubule dilation,inflammatory cell infiltration and fibrosis were observed in renal interstitium,and the fibrosis score was significantly increased(P＜0.01),the mucus layer of colon was thinner and the number of goblet cells were reduced,the positive expression of Muc2 in colon tissue were decreased,and the expression levels of Muc2 mRNA and protein in colon tissues were decreased(P＜0.01).Compared with the model group,RS could significantly reduce the levels of serum Scr,BUN,IL-1β,IL-6 and TNF-α in high and low dose groups(P＜0.01),the renal tissue morphology was significantly improved,and the renal fibrosis score was significantly decreased(P＜0.01),the thickness of colon mucus layer increased and the number of goblet cells increased,the positive expression of Muc2 in colon tissue were increased,the expression level of Muc2 mRNA and protein in colon tissues were significantly increased(P＜0.01).Conclusion RS could effectively protect the residual renal function of CRF rats and delay the progression of CRF.The mechanism might be related to the restoration of colonic mucus barrier function and improvement of inflammatory state of CRF rats.
Rheum palmatum-Salvia miltiorrhiza drug pairschronic renal failurecolonic mucus barrierMuc2
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