首页|基于UPLC-Orbitrap-HRMS技术鉴定当归-川芎药对化学成分及大鼠体内入血成分

基于UPLC-Orbitrap-HRMS技术鉴定当归-川芎药对化学成分及大鼠体内入血成分

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目的 鉴定当归-川芎药对化学成分及大鼠ig后的入血原型成分和代谢产物.方法 基于超高效液相色谱-静电场轨道阱-高分辨率质谱技术(UPLC-Orbitrap-HRMS),结合对照品图谱、自建数据库、相关文献信息及Compound Discoverer 3.3、Xcalibur qual browser 4.3等软件对当归-川芎药对的化学成分进行分析鉴定;大鼠经ig给药后制得含药血清,通过比对含药血清与对照组血清,鉴定入血原型成分和代谢产物.结果 共在当归-川芎药对中鉴定出69个化学成分,主要包括苯酞类24个、有机酸类24个、氨基酸类8个、含氮类7个、香豆素类4个、木脂素类1个、黄酮类1个;在含药血清中共鉴定出30个入血原型成分,包括苯酞类15个、有机酚酸类13个、香豆素类2个;鉴定出45个代谢产物,包括32个苯酞类代谢产物,13个有机酚酸类代谢产物.结论 明确了当归-川芎药对的化学成分及入血原型成分和代谢产物.
Identification of chemical components of Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs and absorbed components in serum of rats based on UPLC-Orbitrap-HRMS
Objective To identify the chemical components of Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs and their prototype components into blood and metabolites after oral administration in rats.Methods Based on the ultra-performance liquid chromatography coupled with Orbitrap high-resolution mass spectrometry technology(UPLC-Orbitrap-HRMS),the chemical components of Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs were analyzed and identified in combination with reference substance,self-established database,relevant literature information,Compound Discoverer 3.3,and Xcalibur qual browser 4.3 software;Drug-containing serum were prepared from normal rats by intragastric administration.The prototype components and metabolites were identified by comparing drug-containing serum with blank serum.Results A total of 69 chemical components were identified in the Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs,including 24 phthalides,24 organic acids,eight amino acids,seven nitrogen-containings,four coumarins,one lignan and one flavonoid.A total of 30 prototype components were identified in the drug-containing serum,including 15 phthalides,13 organic acids,and two coumarins;45 metabolites were identified in vivo,including 32 phthalides and 13 organic acids.Conclusion The chemical components,prototype components,and metabolites of Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairs were clarified.

Angelicae Sinensis Radix-Chuanxiong Rhizoma herb pairsserum pharmacochemistryphthalidesorganic acidsamino acidsprototype components into bloodmetabolitesUPLC-Orbitrap-HRMS

陈紫琪、董义伟、胡开银、赵芮、苏友、刘金鑫、孙黎

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安徽中医药大学药学院,安徽合肥 230012

药物制剂技术与应用安徽省重点实验室,安徽合肥 230012

现代药物制剂安徽省工程技术研究中心,安徽合肥 230012

当归-川芎药对 血清药物化学 苯酞类 有机酸类 氨基酸类 入血原型成分 代谢产物 超高效液相色谱-静电场轨道阱-高分辨率质谱

安徽省高等学校自然科学研究项目

KJ2021A0595

2024

10.7501/j.issn.1674-6376.2024.04.011

药物评价研究
天津药物研究院 中国药学会

药物评价研究

CSTPCD北大核心
影响因子：1.199
ISSN：1674-6376
年,卷(期)：2024.47(4)
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