摘要
目的 观察鼻咽清毒颗粒(BQG)对放疗引起大鼠口腔黏膜损伤的保护作用,并探讨其可能的作用机制.方法 采用FAT细胞皮下注射法,建立F344大鼠种植瘤模型.F344大鼠分为对照组、对照放射组、模型组、BQG低、中、高剂量(1、2、4g·kg-1)组.除对照组外,各组动物进行X线照射,放射剂量为10Gy·d-1,连续5d.BQG 3个剂量组于第1天造模后进行药物干预,每天ig给药1次,连续28d;其余3组给予等体积的蒸馏水.每日对各组大鼠进行一般观察,定期进行称重.末次给药后24h处死大鼠,采集各组大鼠口腔黏膜及血浆,酶联免疫吸附实验(ELISA)检测大鼠血浆中白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、肿瘤坏死因 α(TNF-α)水平;Western blotting检测口腔黏膜NF-κB p65、p-P65、MAPK、ERK、p-ERK等蛋白表达情况,并对其进行组织病理学检查.结果 BQG低、中、高剂量组均能提高大鼠体质量增长率;BQG各给药组能显著降低口轻黏膜组织中IL-6、IL-1β、TNF-α水平(P＜0.05、0.01);与模型组比较,BQG各给药组MAPK蛋白表达显著降低,p-p65/p65,p-ERK/ERK比值显著降低(P＜0.05、0.01).结论 BQG对放射性口腔黏膜炎具有修复作用,其可能通过调控NF-κB/MAPK/ERK信号通路,降低口腔黏膜炎症因子水平相关.
Abstract
Objective To observe the protective effect of Biyan Qingdu Granules(BQG)on radiotherapy-induced oral mucosal injury in rats and to explore its possible mechanism of action.Methods An F344 rat implantation tumor model was established by subcutaneous injection of FAT cells.F344 rats were divided into the control group,control radiation group,model group,and BQG low,medium,and high dose(1,2,and 4 g·kg-1)groups.Except for the control group,the animals in each group were subjected to X-ray irradiation at a radiation dose of 10 Gy·d-1 for five consecutive days.Three dose groups of BQG were subjected to pharmacological intervention after the first day of modeling,and the drug was administered by gavage once a day for 28 consecutive days.The remaining three groups were given equal volumes of distilled water.The rats in each group were subjected to general observation daily and weighed periodically.The rats were executed 24 h after the last administration.The oral mucosa and plasma of rats in each group were collected.The enzyme-linked immunosorbent assay(ELISA)detected interleukin-6(IL-6),interleukin 1β(IL-1β),and tumor necrosis factor α(TNF-α)in the plasma of rats.Western blotting detected the oral mucosa NF-κB p65,p-P65,MAPK,ERK,p-ERK,and other protein expression,and histopathological examination.Results The low,medium,and high dose groups of BQG could increase the body weight growth rate of rats;The administration groups of BQG could significantly reduce the levels of IL-6,IL-1 β,and TNF-α in the tissues of the oral light mucous membranes(P＜0.05,0.01,respectively).Compared with the model group,the administration groups of BQG could significantly reduce the protein expression of MAPK,and the ratios ofp-p65/p65,p-ERK/ERK ratio were significantly reduced(P＜0.05,0.01).Conclusion BQG have a therapeutic effect on radioactive oral mucositi,which may be related to reducing the level of oral mucosal inflammatory factors by regulating the NF-κB/MAPK/ERK signaling pathway.
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