Research progress on isolation and storage of exosomes from mesenchymal stem cells
Mesenchymal stem cell derived exosomes(MSCs-Exo)has become a research hotpot in the field of replace Mesenchymal Stem Cell(MSCs)on the therapeutic recently.A good method of exosome separation and storage is the key to ensure the stable quality and repeatability of the obtained exosomes,and is also the premise of clinical transformation.Exosomes have a variety of separation methods,including international gold standard ultrafiltration method,improved sucrose density gradient centrifugation method,ultrafiltration method and microfluidic technology.Storage temperature and storage medium are the main factors affecting the biological integrity of exosomes.This article reviews the current research status of MSCs-Exo and their isolation and storage conditions in order to provide reference for translational research.
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