Antidepressant effect of pure fermented Sojae Semen Praeparatum
Objective A preliminary study on the antidepressant effect of pure-bred fermented Sojae Semen Praeparatum(PF-SSP)and its active components.Methods According to the established process in our laboratory,natural fermented Sojae Semen Praeparatum(NF-SSP)and PF-SSP by Bacillus subtilis were prepared.The quality evaluation of PF-SSP was carried out by colorimetric identification,thin-layer identification,and content determination of flavonoid and γ-aminobutyric acid(GABA).Divided Kunming mice into eight groups randomly by body weight,with 10 mice in each group,half male and half female.The groups were as follows:control group,model group,fluoxetine(positive control,0.01 g·kg1)group,GABA(0.014 45 g·kg-1)group,flavonoid(0.003 05 g·kg-1)group,NF-SSP ethanol extract washout material(water washout material,1.82 g·kg-1)group,NF-SSP total extract(NF-SSP,1.82 g·kg-1)group,PF-SSP total extract group(PF-SSP,1.82 g·kg-1).Except for the control group,an experimental model of chronic mild unpredictable stress(CUMS)in mice was established,and the mice were given drugs while being subjected to the model.Drugs were administered ig one hour before the stress every day for 28 consecutive days.Body weight was measured in mice at 0,7,14,21,and 28 days.The behavioral indicators of the hanging tail,forced swimming,sucrose preference,and open field test were detected 1 h after giving drugs on day 28.Hematoxylin and eosin(HE)staining was performed to observe pathological changes in the hippocampal CA1 area.UPLC-MS/MS was used to identify the main chemical components of the aqueous extract and pure strain fermentation products.Results PF-SSP and NF-SSP had the same color reaction,color spots and GABA content.Compared with model group,the body weight of each treatment group increased significantly at 14,21,and 28 days after stress(P＜0.01),and the immobility time in the tail suspension and forced swimming test were significantly reduced(P＜0.01);the sucrose preference index of GABA group,isoflavone group,PF-SSP group,and water washout group mice were significantly increased(P＜0.05,0.01);the number of grid crossings and standing up in the open field test of fluoxetine hydrochloride group,washout group,GABA group,and PF-SSP group mice were significantly increased(P＜0.05,0.01);the cells in the CA1 region of the hippocampus in each treatment group were arranged tightly and orderly,with smooth edges and normal cell gaps.The behavioral indices of the pure fermentation group and the natural fermentation total extract group were not significantly different from those of the model group.The natural fermentation ethanol extract washout group and the pure fermentation total extract group both contained daidzein,genistein,GABA,and various amino acids.Conclusion PF-SSP had the similar antidepressant effect as NF-SSP,the main components of antidepressant effect may be GABA and isoflavones.
pure fermentedSojae Semen Praeparatumanti-depressionisoflavonesγ-aminobutyric acid
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