Biological heterogeneity of mesenchymal stem cells derived from different sources based on transcriptomics
Objective To investigate the biological heterogeneity of adipose tissue(AD),placental chorionic membrane(HC),placental amniotic membrane(HA)and umbilical cord(UC)derived mesenchymal stem cells(MSCs)based on transcriptome data.Methods MSCs were isolated from human AD,HC,HA and UC,and the expressions of positive markers(CD73,CD90,CD 105)and negative markers(CD 14,CD34,CD45,CD79a,HLA-DR)on the cell surface were detected by flow cytometry.Improved alizarin red staining,oil red O staining and alisin blue staining were used to detect the three-line differentiation ability of the cells.Total RNA was extracted by Trizol method for transcriptome sequencing,and differential gene expression was analyzed by GFOLD(1.1.4).The gene ontology(GO)functional enrichment analysis of differentially expressed genes was performed using the DAVID database.Results In P2 generation of MSCs from different sources,CD73,CD90 and CD 105 were all positively expressed,while CD14,CD34,CD45,CD79a and HLA-DR were all negatively expressed.The cultured MSCs all had the ability of three-line differentiation.Correlation analysis revealed that MSCs derived from neonatal sources(HA,HC,and UC)were more closely related to each other than those derived from adult sources(AD).In functional enrichment analysis,compared to MSCs from AD,those derived from HA and UC showed superior proliferative abilities.AD-derived MSCs demonstrated better differentiation potential and angiogenesis ability.In contrast,UC-derived MSCs supported neuronal development and secreted chemokines and anti-inflammatory factors that regulate the immune environment.Conclusion MSCs derived from different sources possess distinct biological characteristics,suggesting that the optimal choice for clinical applications may vary depending on the source of MSCs.
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