Protective effects and mechanism of total alkaloids of Stephaniae Cepharantha Radix on leukopenia induced by X-ray radiation
Objective To investigate the protective effect and the mechanism of the total alkaloids of Stephaniae Cepharantha Radix on leukopenia induced by X-ray radiation damage in mice.Methods A mouse model of leukopenia was made by radiation method(one-time whole-body irradiation,irradiation dose of 2.5 Gy).Kunming mice were randomly devided into six groups:control group,model group,total alkaloids of Stephaniae Cepharantha Radix low,medium,high dose(1.25,2.50,5.00 mg·kg-1)groups,and granulocyte colony-stimulating factor(G-CSF)positive control group with 15 animals in each group.Except for the control group,all groups of mice were subjected to one-time whole-body irradiation,and the total alkaloids of Stephaniae Cepharantha Radix were ig administered consecutively for 15 d before and after irradiation in each group,respectively,while the G-CSF group was sc administered for 3 d before irradiation and ig for 15 d after irradiation.The number of nucleated cells and DNA level were calculated after the experiment.Liver,spleen and thymus mass were weighed to calculate organ indices after execution of mice.White blood cells(WBC)in peripheral blood cells were counted before and 3,7 15 d after,respectively,by using a hematology analyzer.Serum from mice were collected by enzyme-linked ELISA for granulocyte-macrophage colony-stimulating factor(GM-CSF),serum interferon(IFN-γ),interleukin-3(IL-3),interleukin-4(IL-4),vascular cell adhesion molecule 1(VCAM-1)and soluble intercellular adhesion factor(SICAM-1).Pathologic changes in the liver and spleen of mice were detected by hematoxylin and eosin staining.The ultrastructure of spleen and liver was observed by electron microscopy.Liver tissue was obtained and tested for antioxidant-related indices.Result It showed that the total alkaloids of Stephaniae Cepharantha Radix could significantly inhibit the reduction of peripheral blood leukocytes within 14 d after X-ray irradiation,at the same time increase the content of bone marrow DNA and the content of hematopoietic growth factor GM-CSF in mice after X-ray radiation,increase the content of immunoinflammatory factors IL-3 and SICAM-1 after X radiation,reduce the damage of liver and spleen tissues,maintain the normal function of the liver and spleen in mice,and significantly reduce the levels of malondialdehyde(MDA)and xanthine oxidase(XOD)in liver.Conclusion Enhancement of immune and hematopoietic functions by the total alkaloids of Stephaniae Cepharantha Radix reveals its therapeutic potential as a radioprotective and radiation mitigating agent.
total alkaloids of Stephaniae Cepharantha Radixleukopeniaionizing radiation damageantioxidantmalondialdehydexanthine oxidase
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