Probing chemical composition of Pheretima guillelmi extracts based on UPLC-Q-Exactive-MS and various techniques
Objective To explore and study the chemical composition and the content of protein,free amino acid,lipid,total sugar,many elements,moisture,ash and ammonium sulphate in the extract of Pheretima guillelmi.Clarify its main material composition and promote the development and application of its preparations.Methods UPLC-Q-Exactive-MS technique was used to investigate the chemical composition of the extract of P.guillelmi;Kjeldahl nitrogen determination was used as an arbitration method for the determination of proteins,Soxhlet extraction was used to determine the content of lipids,and saponification and methylation were used to determine the content of each fatty acid;ICP MS and ICP-OES techniques for the determination of 23 elements;Moisture and total ash content were determined using the drying method and total ash determination method of the Chinese Pharmacopoeia(2020 edition);And ion chromatography for the determination of ammonium sulfate.Results A total of 54 chemical substances were identified,which were categorized into amino acid,nucleoside and organic acid components,of which the content of protein was 53.01％,the content of lipids was 5.62％,the content of free amino acid was 4.18％,the total fatty acid content was 1.23％.Mo,Se,As,V,Mn,Co,Ni,Pb,Sn,Cd,Cr,Cu,Li,B,Fe,Zn,Al,K,Ca,Mg,Na,Hg,and Si were 2.65,4.88,2.31,1.12,25.2,8.32,2.39,1.59,0.057 2,9.27,43.7,27.7,0.642,1.33,759,185,245,614,3.28 × 103,970,1.32 × 103,0.191,and 433 mg·kg-1.Total sugar content was 11.42％,moisture was 4.05％,total ash was 2.36％,and ammonium sulfate content was 9.89％.Conclusion The contents of most of the components in the extract of William's ring worm were basically determined.
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