Evaluation of quality of Valeriana officinalis from different origins based on multi indicator component quantitative combined chemometrics,weighted TOPSIS,and grey relational degree fusion model
Objective To establish a method to evaluate the quality difference of Valeriana officinalis from different producing areas by multi-index component quantitative combined with chemometrics,weighted TOPSIS and grey correlation degree fusion model.Methods Using HPLC to detection the contents of hesperidin,diosmetin,valtrate,acevaltrate,valechlorine,hydroxyvalerenic acid,acetoxyvalerenic acid,valerenic acid,β-sitosterol and ursolic acid in 16 batches of V.officinalis samples with Phenomenex Gemini C18 column,and mobile phase was consisted of acetonitrile-0.05％phosphoric acid solution,gradient elution.The quality difference was comprehensively assessed by chemometrics,weighted TOPSIS and GRA fusion models.Results The validation of HPLC methodology met the requirements of Chinese Pharmacopoeia.The 10 components showed good linearity in their ranges(R2＞0.999),and the average recovery(n=9)were 96.83％-100.08％(RSD＜2.0％).The results of chemometrics analysis showed that 16 batches of Valeriana officinalis were divided into three categories,and acetoxyvalerenic acid,hesperidin,valtrate and hydroxyvalerenic acid were the main markers of the quality differences.The results of weighted TOPSIS and grey correlation degree fusion technology showed that the relative closeness of 16 batches of samples was 0.279 2-0.666 5,and the overall quality of Valerian from Hubei and Guizhou provinces was good(relative closeness was 0.579 0-0.666 5).Conclusion With the content of 10 components such as hesperidin as the evaluation index,the fusion model of chemometrics,weighted TOPSIS and grey relational analysis fusion model can be used to evaluate the quality of Valeriana Officinalis.
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