Pharmacodynamic study of Pulsatilla saponin B4 suppository in treatment of gout in rats
Objective To study the anti-gout effect of Pulsatilla saponin B4 suppository.Methods Pulsatilla saponin B4 suppository was prepared using a mixture of fatty acid glycerides;rats were induced with sodium urate to develop acute gouty arthritis,and the effects of the suppository(9,18,36 mg·kg-1,rectal administration)on the degree of ankle edema were observed,and the levels of inflammatory cytokines monocyte chemotactic protein-1(MCP-1),interleukin-1β(IL-1β),IL-6,and tumor necrosis factor-α(TNF-α)in the serum were detected using a kit,and the pathological changes in the ankle tissue were observed by HE staining;rats were induced with oxalic acid potassium to develop acute hyperuricemia,and allopurinol(a positive control,22 mg·kg-1,ip administration)and the low,medium,and high doses of Pulsatilla saponin B4 suppository(9,18,36 mg·kg-1)were given,and the levels of uric acid,xanthine oxidase(XOD),and blood urea nitrogen(BUN)in the serum were detected using a kit,and the levels of uric acid,BUN,creatinine(CRE),malondialdehyde(MDA),glutathione peroxidase(GSH-Px),and catalase(CAT)in the liver tissue were detected,and the pathological changes in the kidney tissue were observed by HE staining;rats were induced with agarose to establish an inflammatory animal model,and the suppository was observed to affect the degree of edema using a foot volume measurement instrument.Mice were induced with formaldehyde to establish a pain model,and the effect of the suppository(13,26,and 52 mg·kg-1)on the number of licking feet was observed.Results Compared with the model group,Pulsatilla saponin B4 ointment could significantly inhibit the joint swelling induced by sodium urate in acute gouty arthritis rats(P＜0.05,0.01,0.001),reduce the levels of inflammatory factors MCP-1,TNF-α,IL-6,and IL-1β in serum(P＜0.05,0.01,0.001),reduce the levels of serum uric acid,XOD,and BUN in hyperuricemia rats,and reduce the levels of uric acid,BUN,CRE,and MDA in liver,and increased the levels of GSH-Px and CAT in liver(P＜0.05,0.01,0.001),improve the pathological changes of renal tissue;significantly inhibit the paw swelling induced by carrageenan(P＜0.05),significantly reduce the licking foot frequency induced by formaldehyde in mice(P＜0.01).Conclusion Pulsatilla saponin B4 suppository had anti-gouty arthritis,acute hyperuricemia,anti-inflammatory and analgesic effects.
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